A piezoresistive microcantilever immunosensor and its dynamics analysis

LIU Zhi-wei; TONG Zhao-yang; MU Xi-hui; LIU Bing; HAO Lan-qun; ZHANG Jin-ping

doi:10.13873/j.1000-97872014.03.029

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A piezoresistive microcantilever immunosensor and its dynamics analysis

更新时间：2025-09-11

- A piezoresistive microcantilever immunosensor and its dynamics analysis
- Vol. 33, Issue 3, Pages: 8-11(2014)
- 作者机构：
  
  防化研究院国民核生化灾害防护国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872014.03.029
  CLC： TP212.3
- Published：2014
- 稿件说明：
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[1]刘志伟,童朝阳,穆晞惠,刘冰,郝兰群,张金平.一种压阻式微悬臂梁免疫传感器及其动力学分析[J].传感器与微系统,2014,33(03):8-11.

LIU Zhi-wei, TONG Zhao-yang, MU Xi-hui, et al. A piezoresistive microcantilever immunosensor and its dynamics analysis[J]. 2014, 33(3): 8-11.
[1]刘志伟,童朝阳,穆晞惠,刘冰,郝兰群,张金平.一种压阻式微悬臂梁免疫传感器及其动力学分析[J].传感器与微系统,2014,33(03):8-11. DOI： 10.13873/j.1000-97872014.03.029.

LIU Zhi-wei, TONG Zhao-yang, MU Xi-hui, et al. A piezoresistive microcantilever immunosensor and its dynamics analysis[J]. 2014, 33(3): 8-11. DOI： 10.13873/j.1000-97872014.03.029.

摘要

针对传统光学读出微悬臂梁传感器所需测量系统复杂的局限

将生物素—亲和素系统的放大效应与压阻式微悬臂梁传感技术结合起来

成功构建了一种读出方式简单的压阻式微悬臂梁免疫传感器。利用构建的传感器对相思子毒素进行检测

检测限达8μg/L

反应在20 min内基本完成

具有很好的特异性和重现性

能满足水样、土壤、食品等实际模拟样品检测的要求。建立了压阻式微悬臂梁免疫传感器检测相思子毒素的反应动力学模型

并对实际检测数据进行了拟合分析

相关系数R值在0.971 1以上（P<0.001）。根据拟合方程求出的传感器对不同浓度相思子毒素反应达到平衡的响应电压ΔU e、响应时间t0均与实测值非常接近。

Abstract

In view of limitation of traditional optical readout microcantilever sensor which requires complex measuring systems

successfully develop a simple readout piezoresistive microcantilever immunosensor which based on amplification effect of biotin-avidin system and piezoresistive microcantilever sensing technology.Use the constructed piezoresistive microcantileve immunosensor to detect abrin

the reaction can be finished within 20 min and the detection limit is 8 μg / L

with good specificity and reproducibility.The sensor is satisfied with the requirements of simulated samples such as water

soil

food and so on.A reaction kinetics model for the detection of abrin by piezoresistive microcantileve immunosensor is constructed to fit the actual testing datas.Results show that the correlation coefficient R is greater than or equal to 0.971 1（ P < 0.001）.Response voltage ΔU e and response time t 0 obtained from the fitting equation on different concentrations of abrin are very approximate to the measured values.

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references

受体生化药理学[M]. 人民卫生出版社 , 周廷冲编著, 1985

A self-bended piezoresistive microcantilever flow sensor for low flow rate measurement [J] . Qi Zhang,Wenzhou Ruan,Han Wang,Youzheng Zhou,Zheyao Wang,Litan Liu. Sensors & Actuators: A. Physical . 2010 (2)

DNA hybridization measurement by self-sensing piezoresistive microcantilevers in CMOS biosensor [J] . S.M. Yang,C. Chang,T.I. Yin,P.L. Kuo. Sensors & Actuators: B. Chemical . 2007 (2)

Detection of formaldehyde vapor using mercaptophenol-coated piezoresistive cantilevers [J] . Hyejung Seo,Sunghye Jung,Sangmin Jeon. Sensors & Actuators: B. Chemical . 2007 (2)

Investigation of biotin–streptavidin binding interactions using microcantilever sensors [J] . Wenmiao Shu,Ernest D. Laue,Ashwin A. Seshia. Biosensors and Bioelectronics . 2006 (9)

Novel electrical detection of label-free disease marker proteins using piezoresistive self-sensing micro-cantilevers [J] . Kyung Wook Wee,Ghi Yuun Kang,Jaebum Park,Ji Yoon Kang,Dae Sung Yoon,Jung Ho Park,Tae Song Kim. Biosensors and Bioelectronics . 2004 (10)

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Related Institution

College of Science,Naval University of Engineering

Department of Training,Naval University of Engineering

2.Collaborative Research Center,Shanghai University of Medicine and Health Sciences

School of Health Science and Engineering,University of Shanghai for Science and Technology

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