Detection of ischemia modified albumin using SPR biosensor

LI Guang; CHEN Long-cong; LI Xian; YANG Meng; CHEN Meng-meng; XIONG Xing-liang

doi:10.13873/j.1000-97872014.01.002

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Detection of ischemia modified albumin using SPR biosensor

更新时间：2025-09-11

- Detection of ischemia modified albumin using SPR biosensor
- Vol. 33, Issue 1, Pages: 144-146+156(2014)
- 作者机构：
  
  1. 重庆医科大学生物医学工程实验室
  2. 重庆大学生物力学与组织工程教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872014.01.002
  CLC： Q51;TP212.9
- Published：2014
- 稿件说明：
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[1]李广,陈龙聪,李贤,杨萌,陈萌梦,熊兴良.利用SPR生物传感器检测缺血修饰白蛋白[J].传感器与微系统,2014,33(01):144-146+156.

LI Guang, CHEN Long-cong, LI Xian, et al. Detection of ischemia modified albumin using SPR biosensor[J]. 2014, 33(1): 144-146+156.
[1]李广,陈龙聪,李贤,杨萌,陈萌梦,熊兴良.利用SPR生物传感器检测缺血修饰白蛋白[J].传感器与微系统,2014,33(01):144-146+156. DOI： 10.13873/j.1000-97872014.01.002.

LI Guang, CHEN Long-cong, LI Xian, et al. Detection of ischemia modified albumin using SPR biosensor[J]. 2014, 33(1): 144-146+156. DOI： 10.13873/j.1000-97872014.01.002.

摘要

缺血修饰白蛋白（IMA）的检测对心肌缺血的早期诊断和治疗具有重要意义。利用纳米金颗粒和混合巯基自组装于金膜表面上

研制了一种快速检测IMA的表面等离子体共振（SPR）生物传感器。同时比较了直接法和抑制法的检测下限。结果表明:直接检测法可以检测到393ng/L的IMA

而抑制检测法检测限小于5.0 ng/L。与现有的IMA检测方法相比

SPR生物传感器具有特异性好、检测下限低以及检测耗时短等优点。

Abstract

The detection of Ischemia modified albumin（IMA） is significant for early diagnosis and intervention of myocardial ischemia patient. Present a sensitive IMA detection protocol based on surface plasmon resonance（SPR） biosensor combined nanogold and mixed self-assembled Au film. Different assay methods are investigated and compared. Result shows that the limit of detection（LOD） is 393 ng / L for direct detection

while

as to inhibitive assay

the LOD is less than 5. 0 ng / L. Compared with other methods

SPR biosensor exhibits good repetition

in this way

the sensitivity and discrimination of the proposed biosensor are greatly improved.

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references

表面等离子共振技术测定生长抑素含量 [J]. 高锦航,刘瑞,童欢,李献,强鸥,唐承薇. 高等学校化学学报 . 2010(07)

利用液晶取向变化的光学免疫检测方法 [J]. 熊兴良,蔡绍皙,李苑. 高等学校化学学报 . 2009(05)

Nanogold probe enhanced Surface Plasmon Resonance immunosensor for improved detection of antibiotic residues [J] . Fátima Fernández,Francisco Sánchez-Baeza,M.-Pilar Marco. Biosensors and Bioelectronics . 2012 (1)

Insignificant role of the N-terminal cobalt-binding site of albumin in the assessment of acute coronary syndrome: discrepancy between the albumin cobalt-binding assay and N-terminal-targeted immunoassay [J] . Bum Jin Oh,Moon-Hyeong Seo,Hak-Sung Kim. Biomarkers . 2012 (5)

Carbon nanotube-assisted enhancement of surface plasmon resonance signal [J] . Eun Gyo Lee,Kyung Mi Park,Jin Young Jeong,Seung Hui Lee,Jung Eun Baek,Hong Weon Lee,Joon Ki Jung,Bong Hyun Chung. Analytical Biochemistry . 2010 (2)

Sensitivity enhancement of SPR assay of progesterone based on mixed self-assembled monolayers using nanogold particles [J] . J. Yuan,R. Oliver,J. Li,J. Lee,M. Aguilar,Y. Wu. Biosensors and Bioelectronics . 2007 (1)

A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia—a preliminary report 1 1 Original Contributions is coordinated by John A. Marx , MD, of Carolinas Medical Center, Charlotte, North Carolina [J] . David Bar–Or,Edward Lau,James V Winkler. Journal of Emergency Medicine . 2000 (4)

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