A novel method for rapidly and accurately analyzing C reactive proteins（ CRP） in blood samples is established by using surface plasmon resonance（ SPR） biosensing technology. Continuous angle-scanning mode is adopted in the Kretchmann-configuration SPR biosensor

and two individual step motors are used to drive rotation of a prism and a photoelectric detector for high-precision analysis of a single sample. Anti-CRP antibody molecules are coated on the surface of the sensor chip

detect and analyze concentration of CRP in blood samples with SPR sensor. SPR and immune turbidimetry methods are used to detect standard CRP sample and 200 blood samples from children（ 7

10 years old

112 men and 88 women） with infectious diseases

and results show that SPR method detects standard sample has wider linear range. In detection of blood samples from children

SPR biosensor can provide a similar result as that from immune turbidimetry method. However

SPR method is quicker

lower consumption

and better repeatability. Thus

SPR biosensing analysis method has outstanding advantages in CRP detection

and may be widely used in clinical detection analysis.