Design and fabrication of PDMS microfluidic chip based on hydrodynamic focusing

GONG Shan1; GUO Tao1; WANG Chun-shui1

doi:10.13873/j.1000-97872013.08.011

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Design and fabrication of PDMS microfluidic chip based on hydrodynamic focusing

更新时间：2025-09-11

- Design and fabrication of PDMS microfluidic chip based on hydrodynamic focusing
- Vol. 32, Issue 8, Pages: 88-91(2013)
- 作者机构：
  
  1. 仪器科学与动态测试教育部重点实验室
  2. 中北大学电子测试技术重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872013.08.011
  CLC： TN492
- Published：2013
- 稿件说明：
移动端阅览
[1]龚珊,郭涛,王春水.基于水力聚焦的PDMS微流芯片的设计与制作[J].传感器与微系统,2013,32(08):88-91.

GONG Shan1, GUO Tao1, WANG Chun-shui1. Design and fabrication of PDMS microfluidic chip based on hydrodynamic focusing[J]. 2013, 32(8): 88-91.
[1]龚珊,郭涛,王春水.基于水力聚焦的PDMS微流芯片的设计与制作[J].传感器与微系统,2013,32(08):88-91. DOI： 10.13873/j.1000-97872013.08.011.

GONG Shan1, GUO Tao1, WANG Chun-shui1. Design and fabrication of PDMS microfluidic chip based on hydrodynamic focusing[J]. 2013, 32(8): 88-91. DOI： 10.13873/j.1000-97872013.08.011.

摘要

研究制作了一种基于水力聚焦效应

可以对多种不同大小细胞进行聚焦排队实验的聚二甲基硅氧烷（PDMS）微流芯片。芯片的制作过程主要包括制作SU—8阳模

然后利用软光刻方法得到PDMS微流沟道

最后与盖片进行键合封装。用COMSOL软件对样本流与鞘流在不同流速比下进行了流体聚焦仿真

得到了样本的聚焦宽度。利用罗丹明B进行了聚焦验证实验

聚焦宽度与仿真基本一致。通过调节流速比

芯片可用于10～60μm细胞的计数与分析

并利用15μm荧光微球进行了微球排队实验

观察到微球以一列的形式排队经过了聚焦沟道。这种微流芯片具有制作方法简便（SU—8阳模可重复使用）、成本低廉、调控方式简单、通用性强等优点。

Abstract

Research and fabricate a PDMS microfluidic chip which can implement the focusing quening experiment on different sizes of cells based on hydrodynamic focusing effect.The fabricating process of the chip is as follows:firstly

fabricate male mold of SU—8;secondly

make the PDMS microfluidic channel using soft photo-lithography;at last

bond and packge with the cover.Fluid focusing simulation is carried out under condition of different flow rate ratio between the sample flow and sheath flow via the COMSOL software and obtain focusing width of sample.Use Rhodamine B to carry out confirmatory experiment of focusing

focusing width is consistent with the simulation.The chip can be used for count and analysis of cells of

0 μm by adjusting the flow rate ratio.And the experiment of queuing of fluorescent microbeads is carried out

it is observed that microbeads pass through the focusing channel in a line.This fabrication method for microfluidic chip have the advantages of simple process（SU—8 male mold can reuse）

low cost

simple adjusting style and versatility.

关键词

Keywords

references

一种基于微流控芯片技术的流式细胞计数系统 [J]. 刘守坤,苏显中,金庆辉,景奉香,赵建龙. 传感器与微系统 . 2009(10)

微流控芯片流式细胞术 [J]. 刘守坤,苏显中,金庆辉,景奉香,赵建龙. 微电子学 . 2009(05)

一种微流控细胞分离芯片及其流场分析 [J]. 杨静,杨军,许蓉,曹毅,胡宁,郑小林. 仪器仪表学报 . 2009(07)

微流控分析芯片[M]. 科学出版社 , 方肇伦等编著, 2003

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