Research on abrin detection by electrochemiluminescence immunosensor

LIU Bing; TONG Zhao-yang; HAO Lan-qun; MU Xi-hui; LIU Wei; HUANG Qi-bin

doi:10.13873/j.1000-97872013.05.034

Chinese

您当前的位置：

首页 >

文章列表页 >

Research on abrin detection by electrochemiluminescence immunosensor

更新时间：2025-09-11

- Research on abrin detection by electrochemiluminescence immunosensor
- Vol. 32, Issue 5, Pages: 28-30+34(2013)
- 作者机构：
  
  防化研究院国民核生化灾害防护国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872013.05.034
  CLC： TP212.3
- Published：2013
- 稿件说明：
移动端阅览
[1]刘冰,童朝阳,郝兰群,穆晞惠,刘威,黄启斌.电化学发光免疫传感器检测相思子毒素研究[J].传感器与微系统,2013,32(05):28-30+34.

LIU Bing, TONG Zhao-yang, HAO Lan-qun, et al. Research on abrin detection by electrochemiluminescence immunosensor[J]. 2013, 32(5): 28-30+34.
[1]刘冰,童朝阳,郝兰群,穆晞惠,刘威,黄启斌.电化学发光免疫传感器检测相思子毒素研究[J].传感器与微系统,2013,32(05):28-30+34. DOI： 10.13873/j.1000-97872013.05.034.

LIU Bing, TONG Zhao-yang, HAO Lan-qun, et al. Research on abrin detection by electrochemiluminescence immunosensor[J]. 2013, 32(5): 28-30+34. DOI： 10.13873/j.1000-97872013.05.034.

摘要

以磁性微球固定相思子毒素多抗制备捕获探针

以三联吡啶钌标记相思子毒素单抗作为发光探针

两者与相思子毒素发生特异性免疫反应形成夹心复合物

成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素

浓度在0.1～1000μg/L范围内与电化学发光强度呈良好的对数线性关系

拟合方程为lg Y=0.763 lg X+0.562（R=0.9903

N=7

P<0.0001）

检出限为0.1μg/L。

Abstract

Polyclonal antibody（PcAb）of abrin is immobilized on magnetic microbeads as capture probe.An efficient approach for abrin detection by electrochemiluminescence（ECL）immunosensor is developed based on specific immunological reaction between immobilized PcAb

abrin and Ru（bpy）2+3-labeled monoclonal antibody（McAb）

which forms sandwich immunocomplex.Then the target abrin is determined by ECL.A good logarithmic linear relationship between ECL intensity and the concentration of abrin at a range of 0.1

000 μg/L is obtained

fitting equation is lg Y=0.763 lg X+0.562（R=0.990 3

N=7

0.000 1）and the detection limit is 0.1 μg/L.

关键词

Keywords

references

基于生物素-亲和素系统的压电免疫传感器检测相思子毒素研究 [J]. 穆晞惠,周志强,童朝阳,刘冰,郝兰群. 分析化学 . 2009(10)

双抗体夹心生物素-亲和素ELISA法检测相思子毒素 [J]. 穆晞惠,童朝阳,郝兰群,许秀玲,刘冰,田艳慧. 免疫学杂志 . 2007(05)

双抗体夹心ELISA法检测相思子毒素 [J]. 穆晞惠,童朝阳,郝兰群,许秀玲,刘冰,沈桦. 细胞与分子免疫学杂志 . 2007(08)

Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay （ELISA） for detection of abrin in food samples [J] . Yu Zhou,Xiang-Li Tian,Yan-Song Li,Feng-Guang Pan,Yuan-Yuan Zhang,Jun-Hui Zhang,Xin-Rui Wang,Hong-Lin Ren,Shi-Ying Lu,Zhao-Hui Li,Zeng-Shan Liu,Qi-Jun Chen,Jing-Qiu Liu. Food Chemistry . 2012 (4)

Colloidal Gold-Based Immunochromatographic Test Strip for Rapid Detection of Abrin in Food Samples [J] . Gao, Shan,Nie, Chong,Wang, Junhong,Wang, Jing,Kang, Lin,Zhou, Yang,Wang, Jing-Lin. Journal of Food Protection . 2012 (1)

A colloidal gold probe-based silver enhancement immunochromatographic assay for the rapid detection of abrin-a [J] . Wei Yang,Xiao-bing Li,Guo-wen Liu,Bing-bing Zhang,Yi Zhang,Tao Kong,Jia-jia Tang,Dong-na Li,Zhe Wang. Biosensors and Bioelectronics . 2011 (8)

Ultrasensitive Eletrogenerated Chemiluminescence Immunoassay by Magnetic Nanobead Amplification [J] . MingyueLi,YumingSun,LiChen,LuLi,GuizhengZou,XiaoliZhang,WenruiJin. Electroanalysis . 2010 (3)

Rapid and sensitive immunomagnetic-electrochemiluminescent detection of p53 antibodies in human serum [J] . Guihong Yan,Da Xing,Shici Tan,Qun Chen. Journal of Immunological Methods . 2004 (1)

Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics [J] . E. Terpetschnig,H. Szmacinski,H. Malak,J.R. Lakowicz. Biophysical Journal . 1995 (1)

Views

120

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

A piezoresistive microcantilever immunosensor and its dynamics analysis

A piezoelectric cholera toxin immunosensor based on immunoagglunitation

Research progress of MOFs-based aptamer sensors in detection of tumor markers

Preparation of H₂V₁₂O₃₁/BiOI/FTO photoelectrochemicalsensor and its detection of tetracycline hydrochloride

Method of antibody immobilization on surface of immunosensor chip

Related Author

LIU Zhi-wei

ZHANG Jin-ping

LI Yu1

WANG Yuan-sheng2

ZHU Jin-hua1

YU Hong-wei1

QUE Longbin

Gu Xin

Related Institution

College of Science,Naval University of Engineering

Department of Training,Naval University of Engineering

2.Collaborative Research Center,Shanghai University of Medicine and Health Sciences

School of Health Science and Engineering,University of Shanghai for Science and Technology

School of Chemistry and Chemical Engineering,Northeast Petroleum University

⁰