Detection of sulfonamide residues by surface plasmon resonance biosensor

FU Yun-liang; ZHANG Tie-min; WANG Lin-mao

doi:10.13873/j.1000-97872012.12.043

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Detection of sulfonamide residues by surface plasmon resonance biosensor

更新时间：2025-09-11

- Detection of sulfonamide residues by surface plasmon resonance biosensor
- Vol. 31, Issue 12, Pages: 133-135(2012)
- 作者机构：
  
  海南师范大学物理与电子工程学院
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872012.12.043
  CLC： TP212.3
- Published：2012
- 稿件说明：
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[1]符运良,张铁民,王林茂.表面等离子共振生物传感器检测磺胺类药物残留[J].传感器与微系统,2012,31(12):133-135.

FU Yun-liang, ZHANG Tie-min, WANG Lin-mao. Detection of sulfonamide residues by surface plasmon resonance biosensor[J]. 2012, 31(12): 133-135.
[1]符运良,张铁民,王林茂.表面等离子共振生物传感器检测磺胺类药物残留[J].传感器与微系统,2012,31(12):133-135. DOI： 10.13873/j.1000-97872012.12.043.

FU Yun-liang, ZHANG Tie-min, WANG Lin-mao. Detection of sulfonamide residues by surface plasmon resonance biosensor[J]. 2012, 31(12): 133-135. DOI： 10.13873/j.1000-97872012.12.043.

摘要

利用表面等离子共振光学生物免疫传感器

在金膜芯片表面固定抗原技术

实现了磺胺二甲嘧啶药物的定量检测。介绍了抗原分子膜的生长方法

当配置样品中磺胺二甲嘧啶浓度分别为0

1.2

2.4

11.5 mg/L

与工作浓度的抗体混合

测量了传感的信号强度值

得到抑制标准曲线。在无抗牛奶中添加系列质量浓度的磺胺二甲嘧啶样品

利用免疫竞争抑制原理构建标准曲线

检测的浓度线性范围为3～48 mg/L

对浓度为40 mg/L的样品

检测的相对标准偏差小于10%

回收率为99%。检测结果表明:基于表面等离子共振的光学生物免疫传感器在磺胺二甲嘧啶药物残留方面具有很好的应用前景。

Abstract

The surface plasmon resonance（SPR） optical bio-immune sensor is used for detecting the sulfamethazine residues

and it is based on the binding value between monoclonal antigen and antibody of sulfamethazine on the sensor chip surface

which can be used for the capability of quantitative detection for sulfamethazine.The binding values are measured as the concentrations of sulfamethazine are 0

1.2

2.4

11.5 mg/L.The standard curve for sulfamethazine residue in milk is established

and which the linear range for samples is 3

8 mg/L.The relative standard deviation is less than 10 % and percent recovery is 99 % for 40 mg/L concentration.It is sure that the optical bio-immune sensor based on the SPR can be applied in detecti

on of sulfamethazine residues in milk.

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references

利用表面等离子共振技术快速检测牛奶中的磺胺甲噁唑 [J]. 周宏敏,欧惠超,姜浩,江海峰,王晓萍,罗昭锋. 食品科学 . 2010(06)

利用表面等离子谐振生物传感器检测磺胺二甲嘧啶的方法研究 [J]. 李辉,崔大付,王于杰,蔡浩原,王军波,郑自攀,陈兴. 食品科学 . 2007(06)

利用表面等离子谐振免疫生物传感器检测动物组织中的兽药残留 [J]. 郭文林. 中国兽药杂志 . 2004(11)

色谱在食品安全分析中的应用[M]. 化学工业出版社 , 王绪卿,吴永宁等编著, 2005

A capacitive immunosensor for detection of cholera toxin [J] . Analytica Chimica Acta . 2009 (2)

MULTIRESIDUE DETERMINATION OF FLUOROQUINOLONE, SULFONAMIDE, TRIMETHOPRIM, AND CHLORAMPHENICOL ANTIBIOTICS IN URBAN WATERS IN CHINA [J] . Peng, Xianzhi,Tan, Jianhua,Tang, Caiming,Yu, Yiyi,Wang, Zhendi. Environmental Toxicology and Chemistry . 2008 (1)

Combination of analytical and microbiological techniques to study the antimicrobial activity of a new active food packaging containing cinnamon or oregano against E. coli and S. aureus [J] . Analytical and Bioanalytical Chemistry . 2007 (5)

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