Cell electrofusion based on straight-line interdigital microelectrode array

ZHENG Xiao-lin1; YAN Jia-wen1; HU Ning1; YANG Jun1; YANG Jing2

doi:10.13873/j.1000-97872012.02.002

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Cell electrofusion based on straight-line interdigital microelectrode array

更新时间：2025-09-11

- Cell electrofusion based on straight-line interdigital microelectrode array
- Vol. 31, Issue 2, Pages: 23-25+29(2012)
- 作者机构：
  
  1. 重庆大学生物工程学院
  2. 重庆通信学院军事信息工程系
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872012.02.002
  CLC： Q813.2
- Published：2012
- 稿件说明：
移动端阅览
[1]郑小林,鄢佳文,胡宁,杨军,杨静.基于直列式交错微电极阵列的细胞电融合[J].传感器与微系统,2012,31(02):23-25+29.

ZHENG Xiao-lin1, YAN Jia-wen1, HU Ning1, et al. Cell electrofusion based on straight-line interdigital microelectrode array[J]. 2012, 31(2): 23-25+29.
[1]郑小林,鄢佳文,胡宁,杨军,杨静.基于直列式交错微电极阵列的细胞电融合[J].传感器与微系统,2012,31(02):23-25+29. DOI： 10.13873/j.1000-97872012.02.002.

ZHENG Xiao-lin1, YAN Jia-wen1, HU Ning1, et al. Cell electrofusion based on straight-line interdigital microelectrode array[J]. 2012, 31(2): 23-25+29. DOI： 10.13873/j.1000-97872012.02.002.

摘要

提出了一种基于交错式齿状微电极阵列的微流控细胞电融合芯片。利用COMSOL Multiphysics仿真软件

对电场强度有重要影响的微电极几何参数进行了仿真分析

并由此提出了优化的微电极阵列结构。选择SoI硅片的顶层低阻硅加工获得了微电极阵列。实验结果表明:该芯片中采用的直列式微通道结构避免了原有芯片存在的转角易堵塞问题。芯片能够在低电压条件下实现细胞排队和融合过程

具有较高的融合效率。

Abstract

An interdigital microelectrode array based cell electrofusion chip is presented.COMSOL Multiphysics software is used to study the effect of the geometry of microelectrodes on the electric field.Based on these results

an optimized microelectrode structure is given.A straight-line microchannel with high intensity microelectrodes is fabricated on the low-resistance layer of a SoI wafer.Experimental results show that the straight-line structure can avoid the cell trapping at the corner of microchannel network.Cell alignment and electrofusion is carried out on this chip

and it has high fusion efficiency.

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references

高通量细胞电融合芯片研究进展 [J]. 曹毅,杨军,胡宁,杨静,夏斌,郑小林. 传感器与微系统 . 2009(03)

基于MEMS细胞电融合芯片的设计与实验研究 [J]. 夏斌,杨军,胡宁,阴正勤,徐海伟,郑小林. 传感器与微系统 . 2009(02)

用于细胞破裂的微流控生物芯片的研制 [J]. 陈兴,崔大付,刘长春,蔡浩原. 分析化学 . 2006(11)

细胞电穿孔电融合电刺激[M]. 天津科学技术出版社 , 汪和睦, 2000

Study of high-throughput cell electrofusion in a microelectrode-array chip [J] . Yi Cao,Jun Yang,Zheng Qin Yin,Hong Yan Luo,Mo Yang,Ning Hu,Jing Yang,Dan Qun Huo,Chang Jun Hou,Zhi Zhong Jiang,Rui Qiang Zhang,Rong Xu,Xiao Lin Zheng. Microfluidics and Nanofluidics . 2008 (5)

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