Fabrication of ECL glucose sensor based on immobilization glucose oxidase on carbon nanotubes modified electrode

ZHANG Ran; YANG Shan-li; MAI Nan-nan; WEI Wan-zhi; LUO Sheng-lian; NIE Zhou

doi:10.13873/j.1000-97872011.12.038

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Fabrication of ECL glucose sensor based on immobilization glucose oxidase on carbon nanotubes modified electrode

更新时间：2025-09-11

- Fabrication of ECL glucose sensor based on immobilization glucose oxidase on carbon nanotubes modified electrode
- Vol. 30, Issue 12, Pages: 98-101(2011)
- 作者机构：
  
  湖南大学化学生物传感与计量学国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872011.12.038
  CLC： TP212.3
- Published：2011
- 稿件说明：
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[1]张然,杨善丽,买楠楠,魏万之,罗胜联,聂舟.基于碳纳米管固定葡萄糖氧化酶的ECL葡萄糖传感器的制备[J].传感器与微系统,2011,30(12):98-101.

ZHANG Ran, YANG Shan-li, MAI Nan-nan, et al. Fabrication of ECL glucose sensor based on immobilization glucose oxidase on carbon nanotubes modified electrode[J]. 2011, 30(12): 98-101.
[1]张然,杨善丽,买楠楠,魏万之,罗胜联,聂舟.基于碳纳米管固定葡萄糖氧化酶的ECL葡萄糖传感器的制备[J].传感器与微系统,2011,30(12):98-101. DOI： 10.13873/j.1000-97872011.12.038.

ZHANG Ran, YANG Shan-li, MAI Nan-nan, et al. Fabrication of ECL glucose sensor based on immobilization glucose oxidase on carbon nanotubes modified electrode[J]. 2011, 30(12): 98-101. DOI： 10.13873/j.1000-97872011.12.038.

摘要

将葡萄糖氧化酶（GOD）固定在多壁碳纳米管（MWCNTs）修饰电极（ME）上

GOD催化氧化葡萄糖生成过氧化氢

并使鲁米诺产生电致化学发光（ECL）

据此构建了一种新型ECL葡萄糖传感器。结果表明:CNTs修饰的电极对鲁米诺和H2O2反应具有显著的电催化活性和增敏效果。该传感器对葡萄糖检测的线性范围为0.01～10.0mmol/L

Abstract

Incorporation of multi-walled carbon nanotubes on the electrode is performed and modification of glassy carbon electrode with the prepared nano-hybrid material led to the fabrication of a novel electrode.The modified electrode shows attractive electrocatalytic activity and sensitizing effect on Luminol-H2O2 electrochemiluminescence（ECL） reactions at neutral media.The sensitized Luminol-H2O2 reactions are successfully applied for the ECL determination of glucose.Under the optimal conditions for Luminol-H2O2 system

the ECL signal intensity of luminol is linear with the concentration of glucose in the range of 0.01～10.0 mmol/L

correlation coefficient R=0.999（n=5）

the limits of detection for glucose are 5.0 μmol/L.Excellent electrocatalytic activity

high stability

technically simple and possibility of preparation at short period of time are of great advantages of this glucose biosensor.

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references

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