Research on electrical impedance biosensor for rapid detection of e.coli bacteria

ZHANG Feng1; JIN Yan1; LIU De-meng1; GE Yu-qing1; JIN Qing-hui1; ZHAO Jian-long1

doi:10.13873/j.1000-97872011.10.045

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Research on electrical impedance biosensor for rapid detection of e.coli bacteria

更新时间：2025-09-11

- Research on electrical impedance biosensor for rapid detection of e.coli bacteria
- Vol. 30, Issue 10, Pages: 29-32(2011)
- 作者机构：
  
  1. 中国科学院上海微系统与信息技术研究所传感技术国家重点实验室
  2. 中国科学院研究生院
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872011.10.045
  CLC： TP212.3
- Published：2011
- 稿件说明：
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[1]张凤,金妍,刘德盟,葛玉卿,金庆辉,赵建龙.电阻抗式传感器大肠杆菌快速检测方法研究[J].传感器与微系统,2011,30(10):29-32.

ZHANG Feng1, JIN Yan1, LIU De-meng1, et al. Research on electrical impedance biosensor for rapid detection of e.coli bacteria[J]. 2011, 30(10): 29-32.
[1]张凤,金妍,刘德盟,葛玉卿,金庆辉,赵建龙.电阻抗式传感器大肠杆菌快速检测方法研究[J].传感器与微系统,2011,30(10):29-32. DOI： 10.13873/j.1000-97872011.10.045.

ZHANG Feng1, JIN Yan1, LIU De-meng1, et al. Research on electrical impedance biosensor for rapid detection of e.coli bacteria[J]. 2011, 30(10): 29-32. DOI： 10.13873/j.1000-97872011.10.045.

摘要

基于电阻抗法原理

完成了细菌快速检测阻抗式传感器的前端设计

并对叉指式电极进行工艺实现;合理设计实验方案

选取奈奎斯特曲线斜率表征细菌数量的变化

完成了大肠杆菌生长过程的阻抗研究;对测量结果进行分析

并设计实验验证

完成了细菌生长过程中奈奎斯特斜率变化的理论分析

其结果与理论推导吻合。该数据分析方法降低了对测试电极和测量环境的要求

缩短了细菌测定所需要的时间

可用于微生物的快速检测中。

Abstract

The front-end design of the impedance-based biosensor for rapid detection of bacterial has been completed based on the impedance microbiology.Proper experimental scheme is designed to research the impedance change during the Escherichia coli growth.The slope of Nyquist plots is chosen to characterize the bacterial quantity.The measurement results is analyzed

and experiments are designed to verify it.The changes are explained in the Nyquist slope during the bacterial growth.And the results are in agreement with the theoretical derivation.The analysis method reduces the requirements of microelectrodes and measuremental environment and shortens the time

which can be used for rapid detection of microorganisms.

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Keywords

references

电化学阻抗谱导论[M]. 科学出版社 , 曹楚南,张鉴清著, 2002

Bacteriophage-based biosorbents coupled with bioluminescent ATP assay for rapid concentration and detection of Escherichia coli [J] . O. Minikh,M. Tolba,L.Y. Brovko,M.W. Griffiths. Journal of Microbiological Methods . 2010 (2)

Rapid evaluation of biofilm attachment promoters and biofilm growth orientation using a mini-impedimetric device [J] . Juan Jose Goncalves,Rakesh Govind. Sensors & Actuators: B. Chemical . 2009 (1)

Detection of proteins and bacteria using an array of feedback capacitance sensors [J] . Manav Mehta,Chandra Sekar Hanumanthaiah,Pravin Ajitkumar Betala,Hong Zhang,SaeWeon Roh,William Buttner,William R. Penrose,Joseph R. Stetter,Victor H. Pérez-Luna. Biosensors and Bioelectronics . 2007 (5)

Evaluation of a chromogenic medium for total coliforms and Escherichia coli determination in ready-to-eat foods [J] . R.D González,L.M Tamagnini,P.D Olmos,G.B de Sousa. Food Microbiology . 2003 (5)

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