Detection of DNA hybridization based on gold nanorod

CHEN Qiang1; LI Kai1; ZHOU Yu-xun1; WANG Wen-jing2; XIAO Jun-hua1

doi:10.13873/j.1000-97872011.10.004

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Detection of DNA hybridization based on gold nanorod

更新时间：2025-09-11

- Detection of DNA hybridization based on gold nanorod
- Vol. 30, Issue 10, Pages: 137-140(2011)
- 作者机构：
  
  1. 东华大学生物科学与技术研究所
  2. 上海疾病预防控制中心
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872011.10.004
  CLC： TB383.1
- Published：2011
- 稿件说明：
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[1]陈强,李凯,周宇荀,王文静,肖君华.基于金纳米棒的DNA杂交检测[J].传感器与微系统,2011,30(10):137-140.

CHEN Qiang1, LI Kai1, ZHOU Yu-xun1, et al. Detection of DNA hybridization based on gold nanorod[J]. 2011, 30(10): 137-140.
[1]陈强,李凯,周宇荀,王文静,肖君华.基于金纳米棒的DNA杂交检测[J].传感器与微系统,2011,30(10):137-140. DOI： 10.13873/j.1000-97872011.10.004.

CHEN Qiang1, LI Kai1, ZHOU Yu-xun1, et al. Detection of DNA hybridization based on gold nanorod[J]. 2011, 30(10): 137-140. DOI： 10.13873/j.1000-97872011.10.004.

摘要

金纳米棒具有独特的光学性质与良好的生物适应性

在生物医学领域得到了日益广泛的应用。利用种子生长法

通过改变表面活性剂十二烷基二甲基苄基氯化铵（BDAC）的浓度所制备的8种不同长径比（AR）的金纳米棒

具有不同位置的纵向表面等离子共振（SPR）峰。在金纳米棒和非标记探针DNA的混合盐溶液中加入靶标DNA后检测

发现金纳米棒的纵向SPR峰会发生一定的"蓝移"

并伴有明显的吸光度下降;且研究发现该体系检测下限为纳摩尔水平。

Abstract

Gold Nanorods have been widely used in biomedical field

due to their unique optical properties and excellent biocompatibilities.Eight different aspect ratios（AR）gold nanorods which own different longitudinal surface plasmon resonance modes is described.Gold nanorods are prepared with the seed-mediated growth method with various concentration of benzyldimethylhexadecylammonium chloride（BDAC）.While target DNA is added to the mixed buffer of nonmodified gold nanorods and label-free probe DNA with high ionic strength

the"blue-shifted"of gold nanorods’SPR modes can be detected immediately

and obvious absorbance deductions are observed.This method shows high sensitivity which allows detection of target DNA at a concentration as low as nanomolar range.

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references

基于金纳米棒的生物检测、细胞成像和癌症的光热治疗 [J]. 马占芳,田乐,邸静,丁腾. 化学进展 . 2009(01)

One-step quantitative RT-PCR for the detection of rotavirus in acute gastroenteritis [J] . S.-Q. Zeng,A. Halkosalo,M. Salminen,E.D. Szakal,L. Puustinen,T. Vesikari. Journal of Virological Methods . 2008 (2)

Monodispersed Gold Nanorod‐Embedded Silica Particles as Novel Raman Labels for Biosensing [J] . C.Wang,Y.Chen,T.Wang,Z.Ma,Z.Su. Adv. Funct. Mater. . 2008 (2)

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