The principle of fluorescence detection is discussed and two sets of fluorescence spectrum detecting systems are constructed

one syetem is illuminated with an ordinary light source-Deuterium light and the LED is used as light source in another spectrum measurement system.Some experimental researches are carried out

the results indicate that LED greatly enhances the intensity of fluorescence signals as the excitation light source.It not only conduces to data collection and analysis

but also reduces the volume of the system

it is easy to take and measure.The results indicate that LED as an excitation light source in fluorescence spectroscopy measurement system has good application prospects.