Biosensor for glucose based on electrochemical polarization glassy carbon electrode

ZHANG Wei; ZHEN Sheng-hang; XIE Guo-ming; DIAO Qi-zhi; LUO Peng; ZHENG Jun

doi:10.13873/j.1000-97872011.08.026

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Biosensor for glucose based on electrochemical polarization glassy carbon electrode

更新时间：2025-09-11

- Biosensor for glucose based on electrochemical polarization glassy carbon electrode
- Vol. 30, Issue 8, Pages: 110-112+119(2011)
- 作者机构：
  
  重庆医科大学医学检验系临床检验诊断学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872011.08.026
  CLC： TP212.3
- Published：2011
- 稿件说明：
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[1]张伟,甄生航,谢国明,刁奇智,罗鹏,郑军.电化学极化玻碳电极构建的葡萄糖生物传感器[J].传感器与微系统,2011,30(08):110-112+119.

ZHANG Wei, ZHEN Sheng-hang, XIE Guo-ming, et al. Biosensor for glucose based on electrochemical polarization glassy carbon electrode[J]. 2011, 30(8): 110-112+119.
[1]张伟,甄生航,谢国明,刁奇智,罗鹏,郑军.电化学极化玻碳电极构建的葡萄糖生物传感器[J].传感器与微系统,2011,30(08):110-112+119. DOI： 10.13873/j.1000-97872011.08.026.

ZHANG Wei, ZHEN Sheng-hang, XIE Guo-ming, et al. Biosensor for glucose based on electrochemical polarization glassy carbon electrode[J]. 2011, 30(8): 110-112+119. DOI： 10.13873/j.1000-97872011.08.026.

摘要

在硝酸溶液中电化学极化处理玻碳电极（GCE）

以硫堇（TH）为电子介体

通过金—硫、金—氮共价键作用和静电吸附作用将纳米金（GNPs）、葡萄糖氧化酶（GOD）和辣根过氧化物酶（HRP）修饰于电极上

最后在电极上滴涂Nafion水溶液制备抗干扰膜并固定酶电极

构建了一种新型双酶葡萄糖生物传感器。实验结果表明:电化学极化处理电极提高了生物传感器的性能

传感器响应电流与葡萄糖浓度在1×10-3

8×10-3mol/L范围内有良好的线性关系

R=0.991 7

检出限为5×10-5mol/L。该生物传感器具有良好的灵敏度、抗干扰性和稳定性

可以重复使用。

Abstract

A novel amperometric bienzymatic biosensor for determination of glucose is developed by modifying thionine（TH）film on the electrochemical pretreated glassy carbon electrode（GCE）surface.Gold nanoparticles（GNPs）

glucose oxidase（GOD）and horseridesh peroxidase（HRP）are immolilized by electrostatic adsorption and Au-S

Au-N covalent bond self-assembly.Nafion solution is dropped on the modified electrode to prepare anti-interferential and fixed membrane.The experimental results show that the performance of the biosensor can be improved by the electrochemical polarization glassy carbon electrode in HNO3 solution.A good linear relationship between response current and glucose concentration in the range of 1×10-3

8×10-3mol/L

R=0.991 7

the detection limit is 5×10-5mol/L.The biosensor has high sensitivity

long-term stability

excellent interference immunity and reusability.

关键词

Keywords

references

一种新型唾液sIgA电流型纳米免疫生物传感器的制备 [J]. 王艳,甄生航,郑军,马霄,刘成桂,朱洋,谢国明. 传感器与微系统 . 2010(08)

基于纳米金和硫堇固定酶的过氧化氢生物传感器 [J]. 陈贤光,钱莹,张素娟,邹小勇. 化学学报 . 2007(04)

A novel protocol for covalent immobilization of thionine on glassy carbon electrode and its application in hydrogen peroxide biosensor [J] . Xingyong Xu,Yan Feng,Jingjing Li,Feng Li,Hongjun Yu. Biosensors and Bioelectronics . 2010 (10)

Nanoplated bismuth titanate sub-microspheres for protein immobilization and their corresponding direct electrochemistry and electrocatalysis [J] . Xiaohua Chen,Jianqiang Hu,Zhiwu Chen,Xiumei Feng,Aiqing Li. Biosensors and Bioelectronics . 2009 (12)

Structural studies of electrochemically activated glassy carbon electrode: Effects of chloride anion on the redox responses of copper deposition [J] . Kang Shi,Kun Hu,Sheng Wang,Chung-Yin Lau,Kwok-Keung Shiu. Electrochimica Acta . 2007 (19)

A thionine functionalized multiwalled carbon nanotube modified electrode for the determination of hydrogen peroxide [J] . D.R. Shobha Jeykumari,S. Ramaprabhu,S. Sriman Narayanan. Carbon . 2007 (6)

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