Fabrication of glucose biosensor based on LbL technique modified（PPy/MWCNTs-CS）n enhancement

HUANG Jia-dong1; JU Li-li1; CHEN Jing-jing1; LI Si-yan1; ZHOU Zhong-yan1; HUAI Hong-xia2

doi:10.13873/j.1000-97872011.07.044

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Fabrication of glucose biosensor based on LbL technique modified（PPy/MWCNTs-CS）n enhancement

更新时间：2025-09-11

- Fabrication of glucose biosensor based on LbL technique modified（PPy/MWCNTs-CS）n enhancement
- Vol. 30, Issue 7, Pages: 111-113+115(2011)
- 作者机构：
  
  1. 济南大学医学与生命科学学院
  2. 济南大学化学化工学院
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872011.07.044
  CLC： TP212.3
- Published：2011
- 稿件说明：
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[1]黄加栋,居莉莉,陈敬敬,李思埏,周忠焱,怀红霞.构建基于LbL的(PPy/MWCNTs-CS)n增效的葡萄糖生物传感器[J].传感器与微系统,2011,30(07):111-113+115.

HUANG Jia-dong1, JU Li-li1, CHEN Jing-jing1, et al. Fabrication of glucose biosensor based on LbL technique modified（PPy/MWCNTs-CS）n enhancement[J]. 2011, 30(7): 111-113+115.
[1]黄加栋,居莉莉,陈敬敬,李思埏,周忠焱,怀红霞.构建基于LbL的(PPy/MWCNTs-CS)n增效的葡萄糖生物传感器[J].传感器与微系统,2011,30(07):111-113+115. DOI： 10.13873/j.1000-97872011.07.044.

HUANG Jia-dong1, JU Li-li1, CHEN Jing-jing1, et al. Fabrication of glucose biosensor based on LbL technique modified（PPy/MWCNTs-CS）n enhancement[J]. 2011, 30(7): 111-113+115. DOI： 10.13873/j.1000-97872011.07.044.

摘要

实验层层累积（LbL）多壁碳纳米管（MWCNTs）和壳聚糖（CS）

修饰聚吡咯（PPy）

结合葡萄糖氧化酶（GOD）

构建了葡萄糖生物传感器。实验表明:该传感器对葡萄糖的线性检测范围为3.7×10-4

.123×10-2mol/L

线性相关系数为0.998

检出限为2.4×10-5mol/L。达到1.05×10-6A的稳态电流所用的时间

5 s

于4℃环境保存7 d后峰电流约为原来的95%。传感器抗干扰性强

稳定性好。

Abstract

A glucose biosensor is constructed by immobilizing glucose oxidase（GOD） and polypyrrole（PPy）/multi-walled carbon nanotubes（MWCNTs）-chitosan（CS） on the electrode with layer-by-layer（LbL） technique.Pyrrole is electropolymerized on Pt electrode

then MWCNTs and CS are linked by electrostatic adsorption.GOD is electrostaticly absorbed.The results show the glucose biosensor’s determination range is 3.7×10-4～1.123×10-2 mol/L

a detection limit of 2.4×10-5mol/L

response time is less than 5s.It still keep 95% activity after 7 days.This biosensor exhibits a good anti-interferential quality and an excellent stability.

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references

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