To overcome shortcomings of the conventional continuous-flow PCR microsystems usually need multiple heat sources to provide temperatures for PCR

and the integrated or nonintegrated pumping systems are also required to drive the PCR solution to flow continuously

and thus the PCR is realized.A convective two-temperature PCR microfluidics with a single heat source is studied.The temperatures for this PCR microsystem are formed on the grooved copper flake by the combined fin technology.And

the large temperature differences needed for PCR induce the differences in the flow density

which under certain conditions can be used to transport the PCR solution within a closed-loop channel.The experiment demonstrates that the developed microfluidic PCR can amplify the 112 bp Escherichia coli DNA fragment in 45min.