Design of fluorescence detection device in real-time quantitative PCR instrument

CHENG Qi; KONG Ming; ZHOU Yang

doi:10.13873/j.1000-97872011.03.012

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Design of fluorescence detection device in real-time quantitative PCR instrument

更新时间：2025-09-11

- Design of fluorescence detection device in real-time quantitative PCR instrument
- Vol. 30, Issue 3, Pages: 111-113+117(2011)
- 作者机构：
  
  中国计量学院计量测试工程学院
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872011.03.012
  CLC： TH744.16
- Published：2011
- 稿件说明：
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[1]程琦,孔明,周洋.实时荧光定量PCR仪中荧光检测装置的设计[J].传感器与微系统,2011,30(03):111-113+117.

CHENG Qi, KONG Ming, ZHOU Yang. Design of fluorescence detection device in real-time quantitative PCR instrument[J]. 2011, 30(3): 111-113+117.
[1]程琦,孔明,周洋.实时荧光定量PCR仪中荧光检测装置的设计[J].传感器与微系统,2011,30(03):111-113+117. DOI： 10.13873/j.1000-97872011.03.012.

CHENG Qi, KONG Ming, ZHOU Yang. Design of fluorescence detection device in real-time quantitative PCR instrument[J]. 2011, 30(3): 111-113+117. DOI： 10.13873/j.1000-97872011.03.012.

摘要

设计了基于LED光源的积分球式聚合酶链式反应（PCR）荧光检测装置。系统采用单色LED作为光源

经过积分球的均匀辐射后

减少光源对样品的影响

再通过光电倍增管与后续电路

得到PCR循环中的实时荧光光强

这有效地减小了荧光检测装置的体积

提高了仪器的性价比。实验通过装置和iCycler-iQ5实时荧光PCR仪的检测数据进行比对

仪器偏差分别为9.44%和25.98%

发现装置具有更高的精确度

更好的稳定性和重复性。

Abstract

PCR fluorescence detection device is designed based on LED and integrating sphere.System used monochromatic LED as the light source

which through the integrating sphere of uniform radiation to reduce the influence of light source on the sample.Through the photomultiplier tube and follow-up circuit

the real-time PCR cycle fluorescence intensity is gotten.This design could effectively reduced the volume of fluorescence detection device and improved the cost-effective of instrument.This experimental results is compared with the results of iCycler-iQ5 PCR instrument.The instrumental deviations are 9.44 % and 25.98 % respectively.It shows that this device has higher precision

better stability and reproducibility.

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references

光电倍增管的结构与性能研究 [J]. 江华,周媛媛. 舰船电子工程 . 2009(01)

使用光纤实现荧光定量PCR检测 [J]. 刘志华. 中国测试技术 . 2006(03)

积分球的系统误差分析 [J]. 汤顺青,朱正芳. 计量技术 . 2005(12)

聚合酶链式反应（PCR）荧光检测研究 [J]. 吴坚,曹文祺. 计量学报 . 2002(02)

实时荧光PCR技术[M]. 人民军医出版社 , 李金明, 2007

荧光分析法[M]. 科学出版社 , 许金钩,王尊本主编, 2006

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