Detection of ampicillin residues in milk using SPR biosensor

SU Yang; ZHANG Wan-jie; XU Ke-xin; LIU Jin; SHI Ting

doi:10.13873/j.1000-97872010.11.005

Chinese

您当前的位置：

首页 >

文章列表页 >

Detection of ampicillin residues in milk using SPR biosensor

更新时间：2025-09-11

- Detection of ampicillin residues in milk using SPR biosensor
- Vol. 29, Issue 11, Pages: 40-42+47(2010)
- 作者机构：
  
  天津大学精密测试技术及仪器国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872010.11.005
  CLC： TS252.7
- Published：2010
- 稿件说明：
移动端阅览
[1]苏洋,张婉洁,徐可欣,刘瑾,石婷.SPR生物传感器检测牛奶中氨苄青霉素残留[J].传感器与微系统,2010,29(11):40-42+47.

SU Yang, ZHANG Wan-jie, XU Ke-xin, et al. Detection of ampicillin residues in milk using SPR biosensor[J]. 2010, 29(11): 40-42+47.
[1]苏洋,张婉洁,徐可欣,刘瑾,石婷.SPR生物传感器检测牛奶中氨苄青霉素残留[J].传感器与微系统,2010,29(11):40-42+47. DOI： 10.13873/j.1000-97872010.11.005.

SU Yang, ZHANG Wan-jie, XU Ke-xin, et al. Detection of ampicillin residues in milk using SPR biosensor[J]. 2010, 29(11): 40-42+47. DOI： 10.13873/j.1000-97872010.11.005.

摘要

将表面等离子体共振（SPR）传感器的高灵敏性与抗原抗体的高亲和性相结合

通过自组装分子技术将抗生素的单克隆抗体固定到传感器金膜表面

用于特异性吸附牛奶中的抗生素

实现了抗生素残留的快速、痕量检测。由于牛奶的成分复杂

在测量过程中牛奶中的某些成分产生的非特异性固定引入了极大的测量误差。因此

对牛奶进行了脱脂和稀释预处理

通过实验比对发现

对牛奶进行脱脂并稀释4倍后

其产生的非特异性固定基本消除

接近了纯水的测量水平。最后

对预处理后的牛奶进行了多浓度测量

并建立了不同浓度的氨苄青霉素牛奶样品的工作曲线。所采用传感器的最低测量限（LOD）达到了1.56μg/L（变异系数为7.8%）

低于欧盟规定的牛奶中氨苄青霉素的最低检出限。

Abstract

A biosensor based on surface plasmon resonance（SPR）was used to detect antibiotic residue in milk quickly and specifically.The monoclonal antibody was immobilized on the gold surface of SPR sensor with the self-assembled-monolayer technique.The sensor possesses high sensitivity due to SPR technology and high specificity due to the affinity between antigen and antibody.In addition to be binded to antibiotic

antibody was also bounded to other components in milk

which is called non-specific binding（NSB）

and will introduce great measurement error.So

the milk was defatted and diluted firstly.The experiment shows that the NSB of milk can be avoided after defatted and diluted 4 times

closing to deionized water.Furthermore

the processed milk samples with different ampicillin concentrations were measured and the work curve was built up.The limit of detection（LOD）of used sensor is 1.56 μg/L with 7.8 % CV.The LOD is lower than the maximum residue limit（MRL） of ampicillin.

关键词

Keywords

references

Flow cytometric immunoassay for sulfonamides in raw milk [J] . Wouter de Keizer,Monique E. Bienenmann-Ploum,Aldert A. Bergwerff,Willem Haasnoot. Analytica Chimica Acta . 2008 (1)

Screening of penicillin residues in milk by a surface plasmon resonance-based biosensor assay: comparison of chemical and enzymatic sample pre-treatment [J] . Valérie Gaudin,Jéhanne Fontaine,Pierre Maris. Analytica Chimica Acta . 2001 (2)

Advances in surface plasmon resonance biosensor analysis [J] . Rebecca L Rich,David G Myszka. Current Opinion in Biotechnology . 2000 (1)

Views

372

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Method of antibody immobilization on surface of immunosensor chip

Two-channel photonic crystal fiber temperature sensor based on surface plasmon resonance

Research on spectral characteristic of surface plasmon resonance of Ag-TiO₂ thin films

Research progress of biosensor for pathogenic microorganism detection in milk

Urinary albumin detection based on surface plasmon resonance sensor

Related Author

SHAO Bin

WANG Chunhao

GONG Ruikun

CUI Chuanjin

LI Jing

ZHANG Xiangjun

WEI Fanghao

MU Hai-wei

Related Institution

School of Electrical Engineering, North China University of Science and Technology

School of Information and Control Engineering,China University of Mining and Technology

College of Electronics Science,Northeast Petroleum University

Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases,North China University of Science and Technology

Tangshan Key Laboratory of Advanced Measurement and Control Technology,North China University of Science and Technology

⁰