Make use of the property of oxide bacterium catalyze the gas at room temperature

and produce the dissolving oxygen

polyvinyl alcohol immobilized methane-oxidizing bacteria combined with the Clark oxide electrode technology is used to design a new type of gas microbial sensor method.PVA-alginate-boric acid cross-linking immobilization of methane-oxidizing bacteria is selected.The methane in the air is catalyzed in PBS

DO which is produced through the Oxygen electrode is electrochemicaled and transported to computer to realize real-time record.The experiment which aims at the gas monitoring of the sensor-based system shows that the microbial sensor can detect the volume fraction of methane gas real-time and accurately.It is a kind of simple

swift and sensitive gas-detecting method.