A piezoelectric cholera toxin immunosensor based on immunoagglunitation

LI Yu1; WANG Yuan-sheng2; ZHU Jin-hua1; YU Hong-wei1

doi:10.13873/j.1000-97872010.08.012

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A piezoelectric cholera toxin immunosensor based on immunoagglunitation

更新时间：2025-09-11

- A piezoelectric cholera toxin immunosensor based on immunoagglunitation
- Vol. 29, Issue 8, Pages: 129-131(2010)
- 作者机构：
  
  1. 海军工程大学理学院
  2. 海军工程大学训练部
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872010.08.012
  CLC： R446.6
- Published：2010
- 稿件说明：
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[1]李瑜,王源升,朱金华,余红伟.基于压电免疫凝聚的霍乱毒素检测[J].传感器与微系统,2010,29(08):129-131.

LI Yu1, WANG Yuan-sheng2, ZHU Jin-hua1, et al. A piezoelectric cholera toxin immunosensor based on immunoagglunitation[J]. 2010, 29(8): 129-131.
[1]李瑜,王源升,朱金华,余红伟.基于压电免疫凝聚的霍乱毒素检测[J].传感器与微系统,2010,29(08):129-131. DOI： 10.13873/j.1000-97872010.08.012.

LI Yu1, WANG Yuan-sheng2, ZHU Jin-hua1, et al. A piezoelectric cholera toxin immunosensor based on immunoagglunitation[J]. 2010, 29(8): 129-131. DOI： 10.13873/j.1000-97872010.08.012.

摘要

考察了压电免疫凝聚方法对霍乱毒素的检测:金电极表面自吸附的霍乱毒素抗体和检测池中自由分布的霍乱毒素抗体在霍乱毒素出现时发生凝聚

形成网状交联结构

改变了溶液体系的密度和粘度

产生频率响应。实验结果验证了压电免疫凝聚原理的正确性。且显示本方法具有操作简便

组装步骤少

对霍乱毒素检测具有较明显的线性范围（2～4 mg/L）和较低的检测限（1.65 mg/L）及较好的选择性。有望用于霍乱毒素的快速检测。

Abstract

A novel piezo-immunoaggregation model for detecting cholera toxin was developed and verified.The network structure

formed by the cholera toxin antibody

directly absorbed by the gold electrode or dispersed in the solution of the detection cell

in the presence of cholera toxin.The density and viscosity of the detection cell is ultimately changed

lead to the frequency change of the oscillating quartz crystal.The experimental result verified that the immunoaggregation method could easily be applied for detecting cholera toxin without too much chemical assembling steps.The developed piezoelectric immunosensor was proved to be capable of quantitatively detecting cholera toxin at the concentration range of 2～4mg/L with a determination limit of 1.65mg/L.

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Keywords

references

3种检测霍乱肠毒素方法的比较研究 [J]. 严寒秋,李峰,刘桂荣,于彦国,唐耀武. 中国卫生检验杂志 . 2005(11)

霍乱毒素 [J]. 杨淑静. 生物技术通讯 . 2000(04)

A QCM immunosensor for Salmonella detection with simultaneous measurements of resonant frequency and motional resistance [J] . Xiao-Li Su,Yanbin Li. Biosensors and Bioelectronics . 2005 (6)

Verwendung von Schwingquarzen zur W?gung dünner Schichten und zur Mikrow?gung [J] . Günter Sauerbrey. Zeitschrift für Physik . 1959 (2)

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