Immobilization of laccase and its application in optical fiber oxygen sensor

ZHOU Ju-ying; XU Liu-su; PAN Rong-jun

doi:10.13873/j.1000-97872007.12.036

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Immobilization of laccase and its application in optical fiber oxygen sensor

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- Immobilization of laccase and its application in optical fiber oxygen sensor
- Issue 12, Pages: 109-111(2007)
- 作者机构：
  
  1. 广西工学院信息与计算科学系
  2. 广西工学院信息与计算科学系,广西,柳州,545006
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872007.12.036
  CLC： TP212
- Published：2007
- 稿件说明：
移动端阅览
[1]周菊英,徐柳苏,盘荣俊.漆酶固定化及其在光纤氧传感器中的应用[J].传感器与微系统,2007(12):109-111.

ZHOU Ju-ying, XU Liu-su, PAN Rong-jun. Immobilization of laccase and its application in optical fiber oxygen sensor[J]. 2007, (12): 109-111.
[1]周菊英,徐柳苏,盘荣俊.漆酶固定化及其在光纤氧传感器中的应用[J].传感器与微系统,2007(12):109-111. DOI： 10.13873/j.1000-97872007.12.036.

ZHOU Ju-ying, XU Liu-su, PAN Rong-jun. Immobilization of laccase and its application in optical fiber oxygen sensor[J]. 2007, (12): 109-111. DOI： 10.13873/j.1000-97872007.12.036.

摘要

以戊二醛作为交联剂

成功地将漆酶固定在四氨基酞菁铜（CuTAPc）-Fe3O4纳米复合粒子上。探讨了固定条件对固定化漆酶活力的影响

结果表明:当以10%的戊二醛与纳米载体交联8 h后

再以2 g/L的漆酶溶液（pH=7）于4℃下固定所得固定化漆酶活力最高。固定化漆酶组装在光纤传感器上制备成性能良好的氧传感器。此传感器检测下限为0.45mg/L

精度为0.01mg/L

响应时间小于30 s。

Abstract

Laccase is succesfully immobilized on copper tetraaminophthalocyanine-ferrorerric oxide（CuTAPc-Fe3O4） nanoparticles composites using glutaradehyde as corss-linking.The immobilization conditions are investigated.The results show that nanoparticles reacted with 10 % glutaradehyde for 8 h and subsequently reacted with 2 g/L laccase in pH=7 phosphate buffer at 4 ℃.The immobilized laccase is used in fabricating optical fiber sensor for detecting dissolved oxygen.The optical fiber oxygen sensor has detecting limit of 0.45 mg/L

detecting precision is 0.01 mg/L and response time is less than 30 s.

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references

戊二醛交联壳聚糖球固定化脲酶的制备 [J]. 梁足培,桑明心,李建,曹长青,王广建. 青岛科技大学学报(自然科学版) . 2006(02)

壳聚糖珠固定化乳糖酶的条件及特性研究 [J]. 姜梅,王善荣. 中国生化药物杂志 . 2006(02)

CuTAPc-Fe 3 O 4 纳米复合粒子及其漆酶固定化研究 [J]. 黄俊,周菊英,肖海燕,龙胜亚,王军涛. 化学学报 . 2005(14)

用热聚法固定指示剂的光纤氧气传感器研究 [J]. 姜德生,陈兴,黄俊. 化学学报 . 2003(08)

Applications of laccases and tyrosinases （phenoloxidases） immobilized on different supports: a review [J] . Nelson Durán,Maria A Rosa,Alessandro D’Annibale,Liliana Gianfreda. Enzyme and Microbial Technology . 2002 (7)

Laccase activity tests and laccase inhibitors [J] . Christian Johannes,Andrzej Majcherczyk. Journal of Biotechnology . 2000 (2)

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