Study on direct potentiometric immunosensor based on 4-hydroxy thiophenol SAMs

FENG Chun-liang; LIU Yuan-yuan; LI Ya-nan; YU Hong-xue; SUN Yue

doi:10.13873/j.1000-97872007.05.015

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Study on direct potentiometric immunosensor based on 4-hydroxy thiophenol SAMs

更新时间：2025-09-11

- Study on direct potentiometric immunosensor based on 4-hydroxy thiophenol SAMs
- Issue 5, Pages: 15-17(2007)
- 作者机构：
  
  1. 辽宁师范大学化学化工学院功能材料化学研究所
  2. 辽宁师范大学化学化工学院功能材料化学研究所,辽宁,大连,116029
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872007.05.015
  CLC： TP212
- Published：2007
- 稿件说明：
移动端阅览
[1]冯春梁,刘媛媛,李亚男,于洪学,孙越.基于对羟基苯硫酚自组膜免疫传感器的研究[J].传感器与微系统,2007(05):15-17.

FENG Chun-liang, LIU Yuan-yuan, LI Ya-nan, et al. Study on direct potentiometric immunosensor based on 4-hydroxy thiophenol SAMs[J]. 2007, (5): 15-17.
[1]冯春梁,刘媛媛,李亚男,于洪学,孙越.基于对羟基苯硫酚自组膜免疫传感器的研究[J].传感器与微系统,2007(05):15-17. DOI： 10.13873/j.1000-97872007.05.015.

FENG Chun-liang, LIU Yuan-yuan, LI Ya-nan, et al. Study on direct potentiometric immunosensor based on 4-hydroxy thiophenol SAMs[J]. 2007, (5): 15-17. DOI： 10.13873/j.1000-97872007.05.015.

摘要

利用自组装技术将对羟基苯硫酚（4—HTH）固定在金片电极上

以1—乙基—3—（3—二甲氨基）碳二亚胺盐酸盐（EDC）和N—羟基硫代琥珀亚胺（NHS）作为活化剂

将羊抗—小鼠IgG以共价键形式固定在对羟基苯硫酚自组装单分子膜修饰的金片电极上

制备了一种免疫传感器。根据抗体与抗原之间特异反应前后引起的电极电位变化

对小鼠IgG进行定量测定。利用循环伏安法分别对裸金片电极、对羟基苯硫酚自组装膜、抗体膜、抗原膜进行了表征。结果表明:该免疫传感器灵敏度高、响应速度快、检测范围宽

其检测下限为0.2μg/L

检测范围为0.2

0μg/L

并可以再生、重复利用

寿命可达10 d左右

Abstract

An immobilization procedure of antibody on planar gold electrode is proposed.A pretreated planar gold electrode is first immersed in 4-hydroxy thiophenol（4—HTH） solution for self assembly through the Au-S covalent bond.Then

antibody（sheep-anti-mouse IgG） is immobilized by means of covalent coupling with the activation of 1-ethyl-3-（3-dimethylamino propyl） carbodiimide hydrochloride（EDC） and N-hydroxysulfosuccinimide（NHS）.The sensitivity of the procedure is high with the detection limit of 0.2 μg/L and a linear range from 0.2

0 μg/L.The immunosensor can be regenerated and the life can keep 10 days.Every immobilized step on planar Au electrode is characterized using cyclic voltammograms

and the result p

roves the immobilizes steps are feasible.

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Keywords

references

抗体在自组装单分子膜上的共价键固定化及其传感性能研究 [J]. 冯春梁,孙越,辛君华. 辽宁师范大学学报(自然科学版) . 2005(02)

高半胱氨酸SAM膜电极的制备及其电化学行为研究 [J]. 王慧云,郁章玉,孙勤枢,王宁,齐丽云. 曲阜师范大学学报(自然科学版) . 2003(01)

自组装单分子膜的结构及其自组装机理 [J]. 杨生荣,任嗣利,张俊彦,张绪寿. 高等学校化学学报 . 2001(03)

一种新型有序超薄有机膜──自组膜 [J]. 李景虹,程广金,董绍俊. 化学通报 . 1995(10)

Regeneration of ethyl parathion antibodies for repeated use in immunosensor: a study on dissociation of antigens from antibodies [J] . V.B. Kandimalla,N.S. Neeta,N.G. Karanth,M.S. Thakur,K.R. Roshini,B.E.A. Rani,A. Pasha,N.G.K. Karanth. Biosensors and Bioelectronics . 2004 (4)

An ultrasensitive and stable potentiometric immunosensor [J] . D Purvis,O Leonardova,D Farmakovsky,V Cherkasov. Biosensors and Bioelectronics . 2003 (11)

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