Studied on interaction of an affinity biosensor against TNF-α/sTNFR-I

LI Jing; WU Bao-yan; HUANG Jia-dong; SHI Hai-bin; CHEN Qiang

doi:10.13873/j.1000-97872006.03.009

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Studied on interaction of an affinity biosensor against TNF-α/sTNFR-I

更新时间：2025-09-11

- Studied on interaction of an affinity biosensor against TNF-α/sTNFR-I
- Issue 3, Pages: 25-27+31(2006)
- 作者机构：
  
  1. 南开大学生命科学学院
  2. 南开大学生命科学学院,天津,300071
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872006.03.009
  CLC： TP212
- Published：2006
- 稿件说明：
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[1]李静,吴宝艳,黄加栋,史海滨,陈强.亲和型生物传感器对TNF-α/sTNFR-I相互作用的研究[J].传感器与微系统,2006(03):25-27+31.

LI Jing, WU Bao-yan, HUANG Jia-dong, et al. Studied on interaction of an affinity biosensor against TNF-α/sTNFR-I[J]. 2006, (3): 25-27+31.
[1]李静,吴宝艳,黄加栋,史海滨,陈强.亲和型生物传感器对TNF-α/sTNFR-I相互作用的研究[J].传感器与微系统,2006(03):25-27+31. DOI： 10.13873/j.1000-97872006.03.009.

LI Jing, WU Bao-yan, HUANG Jia-dong, et al. Studied on interaction of an affinity biosensor against TNF-α/sTNFR-I[J]. 2006, (3): 25-27+31. DOI： 10.13873/j.1000-97872006.03.009.

摘要

应用亲和型生物传感器—Asys生物传感器实时监测不同浓度的肿瘤坏死因子-α（TNF-α）与可溶性肿瘤坏死因子受体-I（sTNFR-I）的亲和反应过程

并应用FASTfit软件对数据进行分析。结果表明:sTNFR-I以2.25 ng/mm2的密度固定于样品池敏感基片表面

TNF-α可与其发生特异性、浓度依赖性亲和反应

应用FASTfit软件进行数据拟合

并计算其反应的动力学常数

其反应符合二级动力学方程

结合速率常数kass=4.21×104L.mol-1.s-1

解离速率常数kd iss=2.12×10-2s-1

则解离平衡常数kD=5.05×10-7mol.L-1

结合平衡常数kA=1.99×106L.mol-1。应用IAsys传感器可实时监测结合和解离反应过程

操作简便、快速

是研究TNF-α和sTNFR相互作用动力学的有效方法。

Abstract

The interaction between different concentrations of tumor necrosis factor-α（TNF-α） and soluble tumors necrosis factor receptor-I（sTNFR-I） is studied by using an affinity biosensor-IAsys biosensor

and the data is analyzed by using the FASTfit program.sTNFR-I is immobilized on the cuvette surface with the density of（2.25 ng/mm

）

TNF-α then bound to immobilized sTNFR-I specifically in a dose-dependent manner.By FASTfit fitting

the responses fitted the biphasic association quite well

and the error is small and random.Kinetic constants for TNF-α binding to sTNFR-I are determined from the plots of k

versus the concentration of TNF-α:k

ass

=（4.21×10

L）·mol

-1

·s

-1

diss

=2.12×10

-2

-1

=5.05×10

-7

mol·L

-1

=1.99×10

L·mol

-1

.IAsys biosensor is able to monitor the binding and dissociating courses in real time with simple and quick operation

which provided an effective assay for kinetic analysis of TNF-α/sTNFR-I interaction.

关键词

Keywords

references

应用压电石英晶体生物传感器研究赤芍801与ET-1间的相互作用 [J]. 李静,吴宝艳,蒋跃绒,殷惠军,黄加栋,陈可冀,陈强. 中国中西医结合杂志 . 2004(08)

心力衰竭患者血清肿瘤坏死因子、白细胞介素6和白细胞介素6受体水平的测定及临床意义 [J]. 陈哲林,许金诚. 广东医学院学报 . 2002(04)

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Related Author

LIN Qing1

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Related Institution

College of Chemistry and Chemical Engineering,Univeisity of Jinan

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