Carbon nanotubes-based glucose biosensor prepared using layer-by-layer technique

YANG Yu~1; SHI Hai-bin~1; WU Bao-yan~1; LI Jing~1; ANZHAI Shun-yi~2; CHEN Qiang~1

doi:10.13873/j.1000-97872006.01.005

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Carbon nanotubes-based glucose biosensor prepared using layer-by-layer technique

更新时间：2025-09-11

- Carbon nanotubes-based glucose biosensor prepared using layer-by-layer technique
- Issue 1, Pages: 13-15+18(2006)
- 作者机构：
  
  1. 南开大学生命科学学院
  2. 日本东北大学大学院药学科
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-97872006.01.005
  CLC： TP212
- Published：2006
- 稿件说明：
移动端阅览
[1]杨钰,史海滨,吴宝艳,李静,安顺一,陈强.层层累积技术制备基于纳米碳管的葡萄糖生物传感器[J].传感器与微系统,2006(01):13-15+18.

YANG Yu~1, SHI Hai-bin~1, WU Bao-yan~1, et al. Carbon nanotubes-based glucose biosensor prepared using layer-by-layer technique[J]. 2006, (1): 13-15+18.
[1]杨钰,史海滨,吴宝艳,李静,安顺一,陈强.层层累积技术制备基于纳米碳管的葡萄糖生物传感器[J].传感器与微系统,2006(01):13-15+18. DOI： 10.13873/j.1000-97872006.01.005.

YANG Yu~1, SHI Hai-bin~1, WU Bao-yan~1, et al. Carbon nanotubes-based glucose biosensor prepared using layer-by-layer technique[J]. 2006, (1): 13-15+18. DOI： 10.13873/j.1000-97872006.01.005.

摘要

以多壁纳米碳管（MWCNTs）为电子媒介体和酶的吸附载体

利用层层累积的自组装技术固定葡萄糖氧化酶（GOx）的多层（MWCNTs/GOx）n复合薄膜修饰电极

制备了一种新型葡萄糖生物传感器。结果表明:传感器对葡萄糖的响应电流值随着MWCNTs/GOx复合薄膜层数的不同而变化

当MWCNTs/GOx复合薄膜的层数为6时

响应电流值达到最大。（MWCNTs/GOx）6复合薄膜修饰的葡萄糖生物传感器对3×10-2mol/L葡萄糖的响应电流为1.63μA

响应时间仅为6.7 s。该生物传感器检测的线性范围为5×10-4

.5×10-2mol/L

最低检测浓度可达0.9×10-4mol/L。

Abstract

Take multi-walled carbon nanotubes（MWCNTs） as electronic intermedia and adsorptive carrier of enzyme

a layer-by-layer self-assemble technique is used to immobilize glucose oxidase（GOx） and form（MWCNTs/GOx）

multilayer films to modify electrode

then a new kind of biosensor is prepared.Results show that the response current to glucose of biosensor is changed with different number of MWCNTs/GOx multilayer films

and it reaches the maximum value when the number of MWCNTs/GOx layers is 6.The response current of the（MWCNTs/GOx）

multilayer films-modified biosensor to 3×10

-2

mol/L glucose is 1.63 μA while the response time is only 6.7 s.The linear range of this biosensor is 5×10

-4

1.5×10

-2

mol/L and the lowest detectable concentration can

even reach at 0.9×10

-4

mol/L.

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Keywords

references

碳纳米管[M]. 机械工业出版社 , 朱宏伟等著, 2003

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