Single-cell protein detection methods can detailedly explain life events

at the same time

it is crucial for detection of small amounts of samples.Different types of single cell protein quantitative detection methods are reviewed

including methods based on traditional techniques such as flow cytometry

mass cytometry

enzyme-linked immunospot assay and capillary electrophoresis

and novel technology based on microfluidic chips.The principles

main advantages and disadvantages of these methods are compared.Microfluidic technology can better manipulate single cells and detect more sensitively

so it has greater potential in single cell protein detection.