Design of fluorescent substance rapid detection system based on LED excitation source

GUO Li-quan; YIN Huan-cai; TIAN Jing-jing

doi:10.13873/j.1000-9787(2017)05-0106-03

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Design of fluorescent substance rapid detection system based on LED excitation source

更新时间：2025-09-11

- Design of fluorescent substance rapid detection system based on LED excitation source
- Vol. 36, Issue 5, Pages: 106-108+112(2017)
- 作者机构：
  
  1. 中国科学院苏州生物医学工程技术研究所
  2. 中国科学院生物医学检验技术重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13873/j.1000-9787(2017)05-0106-03
  CLC： TP274;TS207.3
- Published：2017
- 稿件说明：
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[1]郭立泉,尹焕才,田晶晶.LED激发光源的荧光物质快速检测系统设计[J].传感器与微系统,2017,36(05):106-108+112.

GUO Li-quan, YIN Huan-cai, TIAN Jing-jing. Design of fluorescent substance rapid detection system based on LED excitation source[J]. 2017, 36(5): 106-108+112.
[1]郭立泉,尹焕才,田晶晶.LED激发光源的荧光物质快速检测系统设计[J].传感器与微系统,2017,36(05):106-108+112. DOI： 10.13873/j.1000-9787(2017)05-0106-03.

GUO Li-quan, YIN Huan-cai, TIAN Jing-jing. Design of fluorescent substance rapid detection system based on LED excitation source[J]. 2017, 36(5): 106-108+112. DOI： 10.13873/j.1000-9787(2017)05-0106-03.

摘要

针对食品和生化检测中常见的荧光物质4—甲基伞形酮（4-MU）的检测问题

提出了一种基于荧光分析法的荧光物质快速检测方法

并以LED作为激发光源

设计了便携式高灵敏度的荧光物质快速检测系统

并给出电路实现。对系统与多功能酶标仪进行了对比标定和测试

结果表明:所设计的系统线性度为0.997

检测误差小于1.5%

响应时间小于0.5 s

这对于食品和生化荧光物质检测设备的开发

具有一定的参考意义。

Abstract

As a fluorescent substance

4-methylumbelliferone（ 4-MU） is widespread in food and biochemical tests.According to the detection of 4-MU

a fluorescent substance rapid detection method based on fluorescence analysis is proposed. LED as the excitation light source

a portable and high-sensitivity fluorescent substance rapid detection system is designed and realized. Comparison calibration and experiments are implemented between the system and multifunctional microplate reader. It is found that the linearity of the designed system is 0. 997

and the detection error is less than 1. 5 % and response time is less than 0. 5 s. This research paves a reference for development of food and biochemical fluorescent substance tests device.

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references

香豆素类荧光底物的合成及其在微生物检测中的应用进展 [J]. 吴清平,马延霞,张菊梅,韦献虎. 化工进展 . 2014(09)

基于荧光寿命法的溶解氧检测系统设计 [J]. 郭立泉,张玉钧,殷高方,王志刚. 传感器与微系统 . 2011(10)

荧光分光光度法测定儿童血浆壳三糖苷酶活性的评价 [J]. 蔡春林,周新,易有荣,曲喜英,郑芳,刘松梅. 中华检验医学杂志 . 2006(11)

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