A trajectory planning approach based on corner detection for laser capture microdissection is proposed to improve the microdissection precision of large area. The impact of selection of value point on trajectory planning is studied

curvature scale space（ CSS） corner detection algorithm is improved

an automatic method of B-spline trajectory planning is established. When considered with math model of mobile system and motor constraint parameters optimization. Strategy of compensation control of systematic errors without cumulative errors and with acceleration mutation is given. Simulation and experimental result shows that the proposed method possesses higher precision

without cumulative errors and with the advantages of second-order continuity and high computational efficiency. Thus

this method is suitable for no-specific curve model and the field of trajectory planning with high precision.