Because of the weakness of surface tension in channel of polymer microfluidic chip

the unstable flow shape of liquid front-end will occur

even cause cut off which will seriously affect the results of test. A POCT chip is designed and fabricated

and hydrophilic coating of Ti O

2

method is used to solve the problem. Research result shows that the hydrophilic coating improves the hydroxyl content and enhances hydrophilicity of the material. And the liquid has stable flow shape and velocity in channel of chip after hydrophilic coating. In addition

the hydrophilic film has good quality

and the film thickness can reach the hundred nanometers level which will not affect the microstructures in the channel

and the peak value of c Tn I fluorescence detection is stable.