Research and fabrication of detector of urea enzymes based on rapid and quantitative test reader using immunochromatographic strips

ZHENG Yu; WANG Kan; ZHANG Jing-jing; SHEN Guang-xia; CUI Da-xiang

doi:10.13873/J.1000-9787(2016)10-0097-03

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Research and fabrication of detector of urea enzymes based on rapid and quantitative test reader using immunochromatographic strips

更新时间：2025-09-11

- Research and fabrication of detector of urea enzymes based on rapid and quantitative test reader using immunochromatographic strips
- Vol. 35, Issue 10, Pages: 97-99+102(2016)
- 作者机构：
  
  上海交通大学电子信息与电气工程学院仪器科学与工程系纳米生物医学工程研究所
- 作者简介：
- 基金信息：
- DOI：10.13873/J.1000-9787(2016)10-0097-03
  CLC： R318.6;TP391.41
- Published：2016
- 稿件说明：
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[1]郑宇,王侃,张晶晶,沈广霞,崔大祥.金标试纸条的尿素酶快速定量检测仪研制[J].传感器与微系统,2016,35(10):97-99+102.

ZHENG Yu, WANG Kan, ZHANG Jing-jing, et al. Research and fabrication of detector of urea enzymes based on rapid and quantitative test reader using immunochromatographic strips[J]. 2016, 35(10): 97-99+102.
[1]郑宇,王侃,张晶晶,沈广霞,崔大祥.金标试纸条的尿素酶快速定量检测仪研制[J].传感器与微系统,2016,35(10):97-99+102. DOI： 10.13873/J.1000-9787(2016)10-0097-03.

ZHENG Yu, WANG Kan, ZHANG Jing-jing, et al. Research and fabrication of detector of urea enzymes based on rapid and quantitative test reader using immunochromatographic strips[J]. 2016, 35(10): 97-99+102. DOI： 10.13873/J.1000-9787(2016)10-0097-03.

摘要

研制了一种基于CMOS传感器的胶体金免疫层析试纸条的尿素酶定量检测系统。该系统由硬件和软件两部分组成

硬件部分利用CMOS传感器和条码扫描仪来获取检测图像和病人的个人信息。软件部分除了实现免疫层析试纸条光信号的分析和诊断还实现病人信息采集和建立病人信息数据库等关键功能。该软件采用了k-means聚类算法

实现采集图像的精准分析。通过检测200例尿素酶样品试纸条

验证了设备的稳定性和准确性。该系统体积小、便携性强且具有优秀的续航能力

可用于长时间的临床、野外和社区体检。

Abstract

A new immunochromatographic test reader based on CMOS sensor is presented

which is used for detecting colloidal gold strip quantitatively. This system is made of hardware system and software system. The function of the hardware system is to get the detecting image and personal information with the CMOS sensor and the barcode scanner. In addition to realizing the analysis and diagnosing of optical signal of the test strip

the software system can also collect the patient’s information and build the database to manage patients’ information.A clustering algorithm called k-means is used to process the image

realizing the accurate analysis of the image.200 urea enzymes samples are collected to test the accuracy and the stability of this reader. This reader has a small volume and a good portability and can run for long durations

it possesses advantages for clinical detection

outdoor detection and community examination.

关键词

Keywords

references

Rapid pathogen detection by lateral-flow immunochromatographic assay with gold nanoparticle-assisted enzyme signal amplification [J] . Il-Hoon Cho,Arun Bhunia,Joseph Irudayaraj. International Journal of Food Microbiology . 2015

DSKmeans: A new kmeans-type approach to discriminative subspace clustering [J] . Xiaohui Huang,Yunming Ye,Huifeng Guo,Yi Cai,Haijun Zhang,Yan Li. Knowledge-Based Systems . 2014

A comparative study of efficient initialization methods for the k-means clustering algorithm [J] . M. Emre Celebi,Hassan A. Kingravi,Patricio A. Vela. Expert Systems With Applications . 2013 (1)

Expression and function of arginine-producing and consuming-enzymes in the kidney [J] . Olivier Levillain. Amino Acids . 2012 (4)

Minkowski metric, feature weighting and anomalous cluster initializing in K-Means clustering [J] . Renato Cordeiro de Amorim,Boris Mirkin. Pattern Recognition . 2011 (3)

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