Research on MWNT-Nafion/GNPs modified impedance sensor

HU Xiao-qin; LI Xiao-xia; GAO Lou-jun

doi:10.13873/J.1000-9787(2016)06-0021-03

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Research on MWNT-Nafion/GNPs modified impedance sensor

更新时间：2025-09-11

- Research on MWNT-Nafion/GNPs modified impedance sensor
- Vol. 35, Issue 6, Pages: 21-23(2016)
- 作者机构：
  
  延安大学化学与化工学院
- 作者简介：
- 基金信息：
- DOI：10.13873/J.1000-9787(2016)06-0021-03
  CLC： TP212
- Published：2016
- 稿件说明：
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[1]胡晓琴,李晓霞,高楼军.多壁碳纳米管-Nafion/纳米金构建阻抗传感器研究[J].传感器与微系统,2016,35(06):21-23.

HU Xiao-qin, LI Xiao-xia, GAO Lou-jun. Research on MWNT-Nafion/GNPs modified impedance sensor[J]. 2016, 35(6): 21-23.
[1]胡晓琴,李晓霞,高楼军.多壁碳纳米管-Nafion/纳米金构建阻抗传感器研究[J].传感器与微系统,2016,35(06):21-23. DOI： 10.13873/J.1000-9787(2016)06-0021-03.

HU Xiao-qin, LI Xiao-xia, GAO Lou-jun. Research on MWNT-Nafion/GNPs modified impedance sensor[J]. 2016, 35(6): 21-23. DOI： 10.13873/J.1000-9787(2016)06-0021-03.

摘要

利用多壁碳纳米管（MWNT）—Nafion和纳米金（GNPs）修饰金电极构建了一种简单、灵敏检测人端粒DNA的电化学阻抗传感器。首先将Nafion分散的MWNT滴涂于Au电极表面

再利用电化学沉积法将GNPs沉积到MWNT—Nafion修饰Au电极表面

以GNPs为载体固定人端粒探针DNA制备DNA传感器。在最优实验条件下

将传感器用于人端粒DNA的检测中

结果表明:目标人端粒DNA的线性范围为1.0×10-13

.0×10-11mol/L

检出限（S/N=3）为2.5×10-14mol/L。采用MWNT为基底沉积GNPs修饰电极检测的灵敏度显著提高。

Abstract

A novel electrochemical impedance sensor for determination of human telomere DNA is developed on basis of MWNT-Nafion / gold nanoparticles（ GNPs） modified electrode. The bare Au electrode is first coated with MWNT Nafion films by dropping

and then GNPs are electro chemical deposited onto surface of MWNT-Nafion modified Au（ MWNTs-Nafion / GNPs / Au） electrode. Then DNA is fabricated by using GNPs as carrier to immobilize human telomere DNA. Under the optimized experimental conditions

the sensor is used in detection of human telomere DNA

the result shows that the target human telomere DNA concentration is in the range of 1. 0 ×10- 13

5. 0 × 10- 11 mol / L which has good linear relationship with a detection limit of 2. 5 ×

10- 14 mol / L（ S / N =3）. Selectivity of detection is obviously improved using MWNT as substrate deposited GNPs modified electrode.

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references

交流阻抗法在细胞分析中应用的研究进展 [J]. 赵灵芝,张成孝. 化学通报 . 2015(01)

人细胞端粒DNA复制与长度维持 [J]. 景亚青,赵勇,王峰. 生命科学 . 2014(11)

基于磁性纳米颗粒和金纳米粒子构建DNA电化学生物传感技术 [J]. 王小兰,郑静,陈琛,汤亚泥,张帆,何品刚. 分析科学学报 . 2014(04)

p53抑癌基因电化学阻抗传感器研究 [J]. 杜芳静,胡晓琴,宋珂,李晓霞. 传感器与微系统 . 2015(01)

基于金纳米粒子组装电化学DNA传感器检测p53抑癌基因的研究 [J]. 罗贤文,杜芳静,吴烨,高楼军,李晓霞. 分析化学 . 2013(11)

茜素/石墨烯-壳聚糖修饰玻碳电极应用于人类端粒DNA的测定 [J]. 蒋园园,王坤,许崇正,杨小弟,李卉卉. 分析化学 . 2013(04)

三维金纳米团簇/多壁碳纳米管-Nafion膜修饰电极的电化学制备及血红蛋白的直接电化学 [J]. 赵越,洪波,范楼珍. 化学学报 . 2013(02)

人类端粒DNA的分子识别及其作用机制探讨 [J]. 陈勇,赵传奇,曲晓刚. 中国科学:化学 . 2012(12)

端粒及端粒酶的研究进展 [J]. 任建国,周军,戴尧仁. 生物化学与生物物理进展 . 1999(05)

端粒DNA与细胞的衰老、死亡 [J]. 白丽荣. 生物学杂志 . 1997(02)

Decorating graphene sheets with gold nanoparticles for the detection of sequence-specific DNA [J] . Yuzhong Zhang,Wei Jiang. Electrochimica Acta . 2012

Electrochemical Aptasensor for the Determination of Cocaine Incorporating Gold Nanoparticles Modification [J] . XiaoxiaLi,HonglanQi,LihuaShen,QiangGao,ChengxiaoZhang. Electroanalysis . 2008 (13)

Amperometric third-generation hydrogen peroxide biosensor incorporating multiwall carbon nanotubes and hemoglobin [J] . Honglan Qi,Chengxiao Zhang,Xiaorong Li. Sensors & Actuators: B. Chemical . 2005 (1)

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