J Hong-wei, ZHANG Tao, ZHENG Wei-bo, et al. Confocal microscopy imaging acquisition system for space life science[J]. 2014, 33(2): 134-137. DOI: 10.13873/j.1000-97872014.02.042.
空间生命科学共焦显微图像采集系统
摘要
为了能够满足针对空间活体目标高分辨率、三维观察以及对样品的低损伤长时间观察需求
提出了采用多针孔扫描盘技术的快速共焦显微成像系统。基于多针孔扫描盘的全视场扫描特性
提出以适合空间应用的EMCCD作为图像器件的微弱光采集成像方案
并将系统分为3个功能模块
详细分析每个模块的功能与实现方案
根据设计方案完成原理样机研制。通过实验验证证明:系统能在较弱光照情况下获取较高质量的目标图像。
Abstract
In order to meet requirement of high-resolution and three-dimensional observation and low damage
long time observation on space living target
confocal microscopy imaging system with multipinhole scanning disk technology is put forward.It gives the technical solution of low-light-level imaging system based on EMCCD concerning the plane scanning technology for in vivo observation.The system is divided into three functional modules.Detailed analysis of each module’s function and implementation method are given.The system can obtain high quality image of the sample in low-light-level environment.