基于Hg<sup>2+</sup>诱导DNA双链形成的汞离子检测试纸条研制

杜娟; 娄新徽; 金庆辉; 赵建龙

doi:10.13873/j.1000-97872014.01.041

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基于Hg²⁺诱导DNA双链形成的汞离子检测试纸条研制

设计与制造 | 更新时间：2025-09-11

- 基于Hg²⁺诱导DNA双链形成的汞离子检测试纸条研制
- Research and fabrication of test strip for mercury （Ⅱ） ions detection based on Hg²⁺ -induced formation of DNA double chain
- 传感器与微系统 2014年33卷第1期页码：108-112
- 作者机构：
  
  1. 中国科学院上海微系统与信息技术研究所传感技术联合国家重点实验室
  2. 中国科学院大学
  3. 首都师范大学
- 作者简介：
- 基金信息：
  
  国家科技支撑计划资助项目（2012BAK08B05）;上海市科委资助项目（201101042,11CH—15,11530700800,11391901900）
- DOI：10.13873/j.1000-97872014.01.041
  中图分类号： TP212.3
- 纸质出版：2014
- 稿件说明：
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[1]杜娟,娄新徽,金庆辉,赵建龙.基于Hg~(2+)诱导DNA双链形成的汞离子检测试纸条研制[J].传感器与微系统,2014,33(01):108-112.

DU Juan, LOU Xin-hui, JIN Qing-hui, et al. Research and fabrication of test strip for mercury （Ⅱ） ions detection based on Hg²⁺ -induced formation of DNA double chain[J]. 2014, 33(1): 108-112.
[1]杜娟,娄新徽,金庆辉,赵建龙.基于Hg~(2+)诱导DNA双链形成的汞离子检测试纸条研制[J].传感器与微系统,2014,33(01):108-112. DOI： 10.13873/j.1000-97872014.01.041.

DU Juan, LOU Xin-hui, JIN Qing-hui, et al. Research and fabrication of test strip for mercury （Ⅱ） ions detection based on Hg²⁺ -induced formation of DNA double chain[J]. 2014, 33(1): 108-112. DOI： 10.13873/j.1000-97872014.01.041.

摘要

Hg2+能特异性地与2个胸腺嘧啶碱基（T）共价结合

介导T-T错配形成稳定的T-Hg2+-T结构。基于DNA修饰的纳米金探针研制了一种可检测水溶液中Hg2+浓度的层析试纸条。试纸条包含1条控制线和2条测试线

检测结果在5 min内可见

裸眼可见检测灵敏度为100 nmol/L。金标条阅读仪分析结果在10 nmol/L

0μmol/L范围内具有良好的线性关系

且对常见二价重金属离子Cu2+

Ni2+

Mg2+

Zn2+

Pb2+具有很好的选择性。该试纸条灵敏特异、快捷简单、操作方便、成本低廉

在环境监测和食品安全的现场快速检测中具有很好的应用前景。

Abstract

Hg2+ can specificity combine with two thymdines（T）

through mediate T-T mismatch form stable THg2+ -T structure. A test strip for detection of concentration of Hg2+ in aqueous solution based on DNAfunctionalized AuNps is developed. The test strip which includes a control line and two test lines enabled visual detection of Hg2+ with a detection limit of 100 nmol / L

and the detection results can be obtained within 5 min.According to test strip reader

good linearity is showed by the result at the range of 10 nmol / L

10 μmol / L

and great selectivity is showed for other metal ions such as Cu2+

Ni2+

Mg2+

Zn2+ and Pb2+ . The test strip hold

s great potential for rapid on-site detection of Hg2+ in environmental monitoring and food safety inspection due to its sensitivity

specificity

speed

simplicity

operational convenience

and low cost.

关键词

Keywords

references

Ultrasensitive electrochemical sensor for mercury （II） based on target-induced structure-switching DNA [J] . Danhong Wu,Qing Zhang,Xia Chu,Haibo Wang,Guoli Shen,Ruqin Yu. Biosensors and Bioelectronics . 2009 (5)

Label-free aptamer-based colorimetric detection of mercury ions in aqueous media using unmodified gold nanoparticles as colorimetric probe [J] . Li Li,Baoxin Li,Yingying Qi,Yan Jin. Analytical and Bioanalytical Chemistry . 2009 (8)

Highly Sensitive Detection of Mercury（II） Ions by Fluorescence Polarization Enhanced by Gold Nanoparticles [J] . Bang‐CeYe,Bin‐ChengYin. Angewandte Chemie International Edition . 2008 (44)

Rational Design of “Turn‐On” Allosteric DNAzyme Catalytic Beacons for Aqueous Mercury Ions with Ultrahigh Sensitivity and Selectivity [J] . JuewenLiu,YiLu. Angewandte Chemie . 2007 (40)

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基于Hg2+诱导DNA双链形成的汞离子检测试纸条研制

Research and fabrication of test strip for mercury （Ⅱ） ions detection based on Hg2+ -induced formation of DNA double chain

DOI：10.13873/j.1000-97872014.01.041

摘要

Abstract

关键词

Keywords

references

基于Hg²⁺诱导DNA双链形成的汞离子检测试纸条研制

Research and fabrication of test strip for mercury （Ⅱ） ions detection based on Hg²⁺ -induced formation of DNA double chain