Surface plasmon resonance（SPR）technology is used to detect serum tryptase levels in guinea pigs died from anaphylactic shock.The aim of this study is attempted to provide an objective evidence for diagnosis of anaphylactic shock.Firstly

sixteen healthy guinea pigs are randomly divided into experimental group and control group.For each group animals are sub-grouped into animals of instantaneous death and animals kept at-20 ℃ for three days freezing.Anaphylactic shock is induced by injection of human pooled serum.Anti-TPSAB1 are immobilized on the surface of SPR biosensor chips using self-assembly method

and then SPR is adopted to detect the tryptase in guinea pigs serum.The experiments show that the response value of experimental group of tryptase is significantly higher than the control group.The three days freezing condition does not affect the expression for serum tryptase.Therefore biosensor based on SPR has good application prospect in detection of animal serum tryptase

and expression of detection of tryptase may act as an evidence for diagnosis of anaphylactic shock.