电化学发光免疫传感器检测相思子毒素研究

刘冰; 童朝阳; 郝兰群; 穆晞惠; 刘威; 黄启斌

doi:10.13873/j.1000-97872013.05.034

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电化学发光免疫传感器检测相思子毒素研究

研究与探讨 | 更新时间：2025-09-11

- 电化学发光免疫传感器检测相思子毒素研究
- Research on abrin detection by electrochemiluminescence immunosensor
- 传感器与微系统 2013年32卷第5期页码：28-30+34
- 作者机构：
  
  防化研究院国民核生化灾害防护国家重点实验室
- 作者简介：
- 基金信息：
  
  总装预先研究资助项目（404070205）
- DOI：10.13873/j.1000-97872013.05.034
  中图分类号： TP212.3
- 纸质出版：2013
- 稿件说明：
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[1]刘冰,童朝阳,郝兰群,穆晞惠,刘威,黄启斌.电化学发光免疫传感器检测相思子毒素研究[J].传感器与微系统,2013,32(05):28-30+34.

LIU Bing, TONG Zhao-yang, HAO Lan-qun, et al. Research on abrin detection by electrochemiluminescence immunosensor[J]. 2013, 32(5): 28-30+34.
[1]刘冰,童朝阳,郝兰群,穆晞惠,刘威,黄启斌.电化学发光免疫传感器检测相思子毒素研究[J].传感器与微系统,2013,32(05):28-30+34. DOI： 10.13873/j.1000-97872013.05.034.

LIU Bing, TONG Zhao-yang, HAO Lan-qun, et al. Research on abrin detection by electrochemiluminescence immunosensor[J]. 2013, 32(5): 28-30+34. DOI： 10.13873/j.1000-97872013.05.034.

摘要

以磁性微球固定相思子毒素多抗制备捕获探针

以三联吡啶钌标记相思子毒素单抗作为发光探针

两者与相思子毒素发生特异性免疫反应形成夹心复合物

成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素

浓度在0.1～1000μg/L范围内与电化学发光强度呈良好的对数线性关系

拟合方程为lg Y=0.763 lg X+0.562（R=0.9903

N=7

P<0.0001）

检出限为0.1μg/L。

Abstract

Polyclonal antibody（PcAb）of abrin is immobilized on magnetic microbeads as capture probe.An efficient approach for abrin detection by electrochemiluminescence（ECL）immunosensor is developed based on specific immunological reaction between immobilized PcAb

abrin and Ru（bpy）2+3-labeled monoclonal antibody（McAb）

which forms sandwich immunocomplex.Then the target abrin is determined by ECL.A good logarithmic linear relationship between ECL intensity and the concentration of abrin at a range of 0.1

000 μg/L is obtained

fitting equation is lg Y=0.763 lg X+0.562（R=0.990 3

N=7

0.000 1）and the detection limit is 0.1 μg/L.

关键词

Keywords

references

基于生物素-亲和素系统的压电免疫传感器检测相思子毒素研究 [J]. 穆晞惠,周志强,童朝阳,刘冰,郝兰群. 分析化学 . 2009(10)

双抗体夹心生物素-亲和素ELISA法检测相思子毒素 [J]. 穆晞惠,童朝阳,郝兰群,许秀玲,刘冰,田艳慧. 免疫学杂志 . 2007(05)

双抗体夹心ELISA法检测相思子毒素 [J]. 穆晞惠,童朝阳,郝兰群,许秀玲,刘冰,沈桦. 细胞与分子免疫学杂志 . 2007(08)

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