A miniaturized surface plasmon resonance（SPR）biosensor designed using irregular quadrangular prism is introduced.The structure of this prism is similar to that used in the previous TI Spreeta sensor

but its size and optical properties are optimized.Its optical detecting precision and system integration are also greatly improved.In optical path design

a wide light beam red LED with 630 nm wavelength and linear array CCD with 5000 pixel points are adopted as light source and photoelectric detector

respectively

and its optical detecting effect is prior to the near infrared light source with 830 nm wavelength and the linear array silicon LED with 128 pixel points used in the TI Spreeta sensor.Besides of the optimization of optical path

flowing control module and signal acquisition processing module are integrated to form an integrated and miniaturized SPR detecting device for analysis of the interacting between biological macromolecules.SPR experiments about air

water and ethanol are implemented in this new device

and experimental results show that accurate detection on single sample can be achieved

and the detecting precision of the resonance angle is 0.01°

moreover

the detecting results has high linearity and good stability

and the detecting deviation is less than 0.5 %.