具有3D微纳结构PDMS阵列免疫传感芯片研制

宗羽; 何巧红; 陈恒武

doi:10.13873/j.1000-97872012.09.042

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具有3D微纳结构PDMS阵列免疫传感芯片研制

设计与制造 | 更新时间：2025-09-11

- 具有3D微纳结构PDMS阵列免疫传感芯片研制
- Research and fabrication of PDMS array immunosensing chip with 3D micro /nano structure
- 传感器与微系统 2012年31卷第9期页码：102-104+108
- 作者机构：
  
  浙江大学微分析系统研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（20890020）;浙江省自然科学基金资助项目（Y4100254）
- DOI：10.13873/j.1000-97872012.09.042
  中图分类号： TP212
- 纸质出版：2012
- 稿件说明：
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[1]宗羽,何巧红,陈恒武.具有3D微纳结构PDMS阵列免疫传感芯片研制[J].传感器与微系统,2012,31(09):102-104+108.

ZONG Yu, HE Qiao-hong, CHEN Heng-wu. Research and fabrication of PDMS array immunosensing chip with 3D micro /nano structure[J]. 2012, 31(9): 102-104+108.
[1]宗羽,何巧红,陈恒武.具有3D微纳结构PDMS阵列免疫传感芯片研制[J].传感器与微系统,2012,31(09):102-104+108. DOI： 10.13873/j.1000-97872012.09.042.

ZONG Yu, HE Qiao-hong, CHEN Heng-wu. Research and fabrication of PDMS array immunosensing chip with 3D micro /nano structure[J]. 2012, 31(9): 102-104+108. DOI： 10.13873/j.1000-97872012.09.042.

摘要

介绍了一种简便快速加工微阵列免疫传感芯片的新方法。采用化学刻蚀技术加工具有μm级山脉状起伏和nm级表面粗糙度结构（简称为3D微纳表面）的玻璃阳模

以该阳模为模板浇注法制得表面具有3D微纳表面结构的PDMS基片

再借助于物理吸附

将抗体直接固定于该PDMS表面

形成具有3D微纳结构的PDMS微阵列免疫传感器。利用光学显微镜和原子力显微镜对玻璃阳模和PDMS基片表面形貌进行表征

研究了PDMS表面微纳结构化处理对抗体吸附能力的影响。结果表明:3D微纳结构的PDMS由于具有大的比表面积

能显著增强抗体的吸附能力。将研制所得的3D微纳表面结构的PDMS芯片用于微阵列荧光免疫分析

其灵敏度是平板PDMS的5倍。

Abstract

A simple and rapid method for fabrication of PDMS chip for microarray immunosensors is reported.A glass template with micro mountain-like structure and nanometer-lever roughness（termed as 3D micro /nano surface） is fabricated via chemical etching.PDMS substrates with 3D micro /nano structure are directly replicated by soft lithography against the glass template.Antibodies are immobilized onto the surface of the PDMS substrates via physical adsorption

PDMS microarray immunosensors with 3D micro /nano structure is formed.The topography of both the glass templates and PDMS substrates is characterized by optical micro spectroscope and atomic force microscope（AFM）.The effect of the PDMS surface micro /nano structuring processing on the antibody-adsorbing ability is studied.It is found that antibody-adsorbing capability of the 3D micro /nano surface of PDMS substrates is significantly enhanced due to its large specific surface area.When the prepared PDMS chips with 3D micro /nano surface are applied to microarray fluorescence immunoassay

its sensitivity is 5 times of the flat PDMS chips.

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references

Rapid fabrication of microfluidic chip with three-dimensional structures using natural lotus leaf template [J] . Changchun Liu. Microfluidics and Nanofluidics . 2010 (4-5)

Signal enhancement in a protein chip array using a 3-D nanosurface [J] . So Yeon Kim,Jaeeun Yu,Sang Jun Son,Junhong Min. Ultramicroscopy . 2010 (6)

Fabrication of 1D nanofluidic channels on glass substrate by wet etching and room-temperature bonding [J] . Qiaohong He,Shuang Chen,Yuan Su,Qun Fang,Hengwu Chen. Analytica Chimica Acta . 2008 (1)

Current development in microfluidic immunosensing chip [J] . Terence G. Henares,Fumio Mizutani,Hideaki Hisamoto. Analytica Chimica Acta . 2008 (1)

Protein microarrays: Promising tools for proteomic research [J] . Proteomics . 2003 (11)

Adapting arrays and lab‐on‐a‐chip technology for proteomics [J] . DanielFigeys. Proteomics . 2002 (4)

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