HUANG Jia-dong1, JU Li-li1, CHEN Jing-jing1, et al. Fabrication of glucose biosensor based on LbL technique modified(PPy/MWCNTs-CS)n enhancement[J]. 2011, 30(7): 111-113+115.
HUANG Jia-dong1, JU Li-li1, CHEN Jing-jing1, et al. Fabrication of glucose biosensor based on LbL technique modified(PPy/MWCNTs-CS)n enhancement[J]. 2011, 30(7): 111-113+115. DOI: 10.13873/j.1000-97872011.07.044.
构建基于LbL的(PPy/MWCNTs-CS)n增效的葡萄糖生物传感器
摘要
实验层层累积(LbL)多壁碳纳米管(MWCNTs)和壳聚糖(CS)
修饰聚吡咯(PPy)
结合葡萄糖氧化酶(GOD)
构建了葡萄糖生物传感器。实验表明:该传感器对葡萄糖的线性检测范围为3.7×10-4
1
.123×10-2mol/L
线性相关系数为0.998
检出限为2.4×10-5mol/L。达到1.05×10-6A的稳态电流所用的时间
<
5 s
于4℃环境保存7 d后峰电流约为原来的95%。传感器抗干扰性强
稳定性好。
Abstract
A glucose biosensor is constructed by immobilizing glucose oxidase(GOD) and polypyrrole(PPy)/multi-walled carbon nanotubes(MWCNTs)-chitosan(CS) on the electrode with layer-by-layer(LbL) technique.Pyrrole is electropolymerized on Pt electrode
then MWCNTs and CS are linked by electrostatic adsorption.GOD is electrostaticly absorbed.The results show the glucose biosensor’s determination range is 3.7×10-4~1.123×10-2 mol/L
a detection limit of 2.4×10-5mol/L
response time is less than 5s.It still keep 95% activity after 7 days.This biosensor exhibits a good anti-interferential quality and an excellent stability.