LIU Bao, ZHANG Si-xiang, ZHOU Wei, et al. Research and fabrication of image-forming system of the cell based on LIFD[J]. 2011, 30(5): 113-115. DOI: 10.13873/j.1000-97872011.05.002.
Theory of laser-induced fluorescence detection(LIFD) method for concentration of calcium ion detection is analyzed.Because of superiority in cells development and detection
an image-forming system for concentration detecting of calcium ion in cells based on microfluidic chip using the technology of LIFD is built.On the basis of studies of the technology of microfluidic chips
a microfluidic chip has been designed and manufactured for the detection of calcium concentration.A microscope system
a fast wavelength switching system and a CCD image forming system have also been designed.The images of fluorescence are collected by the image-forming system.Based on this new image-forming system
a new application domain is opened for microfluidic chip and a new method is provided for detecting ion in cells.
Real Time in Situ Confocal Imaging of Calcium Wave in the Perfused Whole Heart of the Rat [J] . Takemitsu Hama,Akiyuki Takahashi,Akira Ichihara,Tetsuro Takamatsu. Cellular Signalling . 1998 (5)