With the property of ethanol oxidizing bacteria consuming ethanol and producing dissolved oxygen

ethanol-oxidizing bacteria oxidized with Clark electrode technique to design a new ethanol micro-sensor detection method.The bacterium which uses ethanol as the sole carbon and energy growth was identified as Methyl bacterium.With the PVA-alginate sol-gel embedding method

it is beneficial to the growth of microbial cells.On the screening and detaching

the ethanol oxidizing bacteria are immobilized.In phosphate buffer catalytic ethanol

through the Clark oxygen electrode pairs of the dissolved oxygen to generate electricity transmitting to the computer-based real-time record.Ethanol micro-sensor sensor system for monitoring the results show that microbial-sensor can detect ethanol concentrations real-time and accurately.It is a simple

rapid

sensitive ethanol detection method.