1. 南开大学生命科学学学院生物活性材料教育部重点实验室
2. 中国科学院深圳先进技术研究院生物医学信息与健康工程学重点实验室
3. 天津工业大学环境与化学工程学院
纸质出版:2010
移动端阅览
[1]苗智颖,李莎,秦霞,王会才,陈强.多壁碳纳米管增效层层自组装构建乙醇生物传感器[J].传感器与微系统,2010,29(12):85-87.
MIAO Zhi-ying1, LI Sha2, QIN Xia1, et al. Multi-walled carbon nanotubes enhanced the sensibility of ethanol biosensor based on layer-by-layer deposition technique[J]. 2010, 29(12): 85-87.
[1]苗智颖,李莎,秦霞,王会才,陈强.多壁碳纳米管增效层层自组装构建乙醇生物传感器[J].传感器与微系统,2010,29(12):85-87. DOI: 10.13873/j.1000-97872010.12.034.
MIAO Zhi-ying1, LI Sha2, QIN Xia1, et al. Multi-walled carbon nanotubes enhanced the sensibility of ethanol biosensor based on layer-by-layer deposition technique[J]. 2010, 29(12): 85-87. DOI: 10.13873/j.1000-97872010.12.034.
以聚二烯丙基二甲基氯化铵(PDDA)为阳离子聚电解质
以乙醇氧化酶(AOD)为带负电的生物分子
结合多壁碳纳米管(MWCNTs)
层层自组装(layer-by-layer)制备聚烯丙胺/聚磺化乙烯硫酸盐乙醇(PAA/PVS)3抗干扰膜修饰Pt电极
在此基础上将PDDA与AOD交替组装在修饰好的电极上
构建了电流型乙醇生物传感器。实验结果表明:MWCNTs的引入使电极对H2O2的催化电流明显增大
制成的酶电极可以有效控制酶量的使用
酶膜组装层数为6时最优
对乙醇的灵敏度为2.913μA/mol/L
在2×10-4
8
×10-3mol/L浓度范围内呈良好线性关系
检出限为1.52×10-5mol/L(S/N=3)
并且传感器具有良好的抗干扰能力和稳定性。
A novel ethanol biosensor of Pt-(PAA/PVS)3-(MWCNTs-PDDA/AOD) 6with enhanced sensibility
excellent anti-interfence property is fabricated.Poly(allylamine)/poly(potassium vinyl sulfate)(PAA/PVS) multiple film is self-assembled on the surface of Pt electrode based on layer-by-layer deposition to enhance the anti-interference ability.Poly(dially dimethyl ammonium) chloride(PDDA) is used as cationic polyelectrolyte and multi-walled carbon nanotubes(MWCNTs) is conformed to be useful in ethanol biosensors fabrication via comparing the biosensors fabricated with and without MWCNTs.It shows a linear response to ethanol in the range of
2.0×10-4
8
.0×10-3mol/L
high sensibility of 2.913 μA/(mmol/L)
low detection limit of 1.52 ×10-5mol/L(S/N=3)
and good suppression of interference ability and stability.
层层自组装制备基于多壁碳纳米管的胆碱生物传感器 [J]. 秦霞,王艳艳,赵紫霞,王新胜,李莎,于敏,黄楠,苗智颖,陈强. 分析化学 . 2008(06)
基于多壁碳纳米管和聚丙烯胺层层自组装的葡萄糖生物传感器 [J]. 尹峰,赵紫霞,吴宝艳,王新胜,王艳艳,陈强. 分析化学 . 2007(07)
层层自组装技术在生物医用材料领域中的应用研究进展 [J]. 林全愧,计剑,谭庆刚,任科峰,沈家骢. 高分子通报 . 2006(08)
层层累积技术制备基于纳米碳管的葡萄糖生物传感器 [J]. 杨钰,史海滨,吴宝艳,李静,安顺一,陈强. 传感器与微系统 . 2006(01)
碳纳米管的功能化研究进展 [J]. 肖素芳,王宗花,罗国安. 分析化学 . 2005(02)
Effects of the type of polycation in the coating films prepared by a layer-by-layer deposition technique on the properties of amperometric choline sensors [J] . Haibin Shi,Zhao Song,Jiadong Huang,Yu Yang,Zixia Zhao,Jun-Ichi Anzai,Tetsuo Osa,Qiang Chen. Sensors & Actuators: B. Chemical . 2005 (2)
Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes [J] . Mustafa Musameh,Joseph Wang,Arben Merkoci,Yuehe Lin. Electrochemistry Communications . 2002 (10)
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