XIANG Fang-xian1, GUO Kang-quan1, HOU Jun-cai1, et al. Study on thin-layer electrode fabrication technology for pesticide residue biosensors[J]. 2009, 28(5): 40-42+45. DOI: 10.13873/j.1000-97872009.05.020.
农药残留生物传感器薄层电极制作工艺研究
摘要
为了开发农药残留生物传感器的薄层电极
研究尝试采用重复银镜反应和阳极氧化法制作参比电极;以1×10-4mol/L对硝基苯酚为测定液
采用时间—电势法对制作参比电极的工艺参数进行优化
确定Ag电极制作工艺是银镜反应次数9次、面积98 mm2
Ag/AgC l电极制作工艺是阳极氧化3 m in、面积70mm2。采用循环伏安法对电极所做的检测结果表明:电极间差异小于5%
具有很好的一致性;Ag电极适用电压为-1.35
1
.15 V
Ag/AgC l电极适用电压为-1.45
1
.0 V。
Abstract
Based on silver mirror reaction and anodic oxidation thin-layer electrode is prepared for pesticide residue biosensors.The thin-layer electrode fabrication technology parameter is optimized by time-potential determination in 1×10-4mol/L p-nitrophenol solution.The best way to made Ag reference electrode is:silver mirror reaction 9 times
surface area of 98 mm2;Ag/AgCl electrode is fabricated by hydrochloric acid concentration anodic oxide 3 min and with the surface area of 70 mm2.Cyclic voltammetry is applied to assess the coherence of prepared thin-layer electrode.The results show that the variation is less than 5 %.The suitable voltage range is:Ag electrode of-1.35