湖北襄樊学院化学与生物科学系
纸质出版:2009
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[1]刘素芹,戴高鹏,王启会.串联电极阵列压电传感技术自动检测病原体[J].传感器与微系统,2009,28(04):66-68+75.
LIU Su-qin, DAI Gao-peng, WANG Qi-hui. Automatic detection of pathogens with separated electrodes series piezoelectric sensing technique[J]. 2009, 28(4): 66-68+75.
[1]刘素芹,戴高鹏,王启会.串联电极阵列压电传感技术自动检测病原体[J].传感器与微系统,2009,28(04):66-68+75. DOI: 10.13873/j.1000-97872009.04.029.
LIU Su-qin, DAI Gao-peng, WANG Qi-hui. Automatic detection of pathogens with separated electrodes series piezoelectric sensing technique[J]. 2009, 28(4): 66-68+75. DOI: 10.13873/j.1000-97872009.04.029.
以串联电极阵列压电石英晶体(SPQC)传感器为基础构建了一台8通道微生物自动检测仪。电极插入培养基检测溶液电导的变化。池常数k=0.05m
使用中性、低电导的氨基酸培养基(AaB)
电导控制灵敏度区间为550
6
00μS。利用拟合—微分的方法简单准确地得到频率检测时间(FDT)。对普通的金黄色葡萄球菌(S.aureus)和痢疾杆菌(S.dysenteriae)进行自动检测
结果表明:FDT和样品中细菌浓度呈线性关系(R>0.99)
检测范围为10
1
06CFU.mL-1。该方法准确度与国标的平板记数法相当(置信区间为95%)
但重现性更好
更稳定
灵敏度更高。
8 independent SPQC sensors is parallel connected to form a muti-channels detecting unit.Electrodes are immersed into culture medium to detect conductivity change of solution.The cell constant k is determined as 0.05m
and the sensitivity interval of the device is from 550 to 600μs.To maintain sensitivity of SPQC sensor
a novel culture medium(a mino acid broth) AaB is developed.FDT is obtained veraciously by using fitting-differentiating method.Common pathogens S.aureus and S.dysenteriae are determined with the automated detecting device and methods mentioned above.Results show that the calibration curves of FDT against density of bacteria show a linear correlation coefficient(R>0.99) over
the range of 10
1
06 CFU·mL-1.Detection results are all inside the 95% confidence interval of standard pour plate counting method.The reproducibility is also reviewed
and the device is still stable and sensitive.
A rapid method for determining Mycobacterium tuberculosis based on a bulk acoustic wave impedance biosensor [J] . Fengjiao He,Jianwen Zhao,Liude Zhang,Xueni Su. Talanta . 2003 (5)
Monitoring of DNA oxidative damage with piezoelectric quartz crystal method [J] . Yaohui Wu,Lin Yi,Qingji Xie,Youyu Zhang,Fan Yin,Shouzhuo Yao. Talanta . 2001 (2)
Monitoring of mutagenic process with piezoelectric quartz crystal impedance analysis [J] . Jinzhong Zhang,Wanzhi Wei,Anhong Zhou,Deliang He,Shouzhuo Yao,Qingji Xie. Talanta . 2000 (3)
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