A kind of NO sensor is developed

and it is used in the vivo measurement of meridian experiments.The nitric oxide sensor is made of the pure platinum wire modified by the chitosan and Ni2+ solution and decorated by the Nafion membranes from its surface.This sensor is used to measure the nitric oxide concentration of the acupoints and non-acupoints before and after acupuncture in the rats.The linear response range of this sensor is 10-6

1

0-4mol/L with the square of the correlation coefficient 0.9848.Reproducibility is 4.72%

average recovery is 97.74%

in the experiments of meridian

the current of NO in the acupoints is obviously higher than in the non-acupoints（P

<

0.05）.Its selectivity

s

tability and repeatability are good and its pintype structure is applicable to vivo measurement.The results in vivo experiments of the rats show that there is the specificity in the acupoints and suggest that there is the existence of meridian specificity

so this sensor can provide a support in the technology for the theory of the meridian and acupoint.