FU Hui-juan, HUANG Hui-ping, CAI Qing-hua. Study on multi-walled carbon nanotubes modified amperemetric galactose biosensor[J]. 2007, (9): 67-69. DOI: 10.13873/j.1000-97872007.09.034.
多壁碳纳米管半乳糖生物传感器研究
摘要
以普鲁士蓝(PB)膜玻碳电极为基质
用多壁碳纳米管固定半乳糖氧化酶(GAO)
构造了半乳糖电流型传感器
研究了半乳糖氧化酶在此传感器中的催化反应机理
对多壁碳纳米管半乳糖传感器的检测条件进行了优化。确认其对半乳糖具有灵敏的生物响应性
表现出良好的线性和分辨力。此传感器对半乳糖摩尔浓度0.5×10-4
1
.5×10-2mol/L呈线性响应
检出限为1.0×10-4mol/L
响应时间为10 s
30 d后
响应电流依然保持80%。
Abstract
An amperometric galactose biosensor is formed based on the enzyme electrode with multi-walled carbon nanotubes(MWNTs) modified on Prussian blue(PB)modified glassy carbon electrode.The bioelectrochemical characterization of galactose oxidase of the galactose biosensor is studied.The experimental conditions related to the characterization of sensor have been studied in detail.It is confirmed that the electrode has sensitive biological response performance
which led to better linearity and resolution.The sensor offeres a linear range of 0.5×10-4
1
.5×10-2mol/L and a detection limit of 1.0×10-4mol/L.Response time is 10 s
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