The mould of the chip is fabricated with the printed circuit board fabrication technique.Based on the mould

the microfluidic chip is fabricated with PDMS material.The chip is composed of base slab and cover slab

and the microchannel with 75 μm in depth and 100 μm in width is laid on the base slab sealed with the cover slab.The electrophoresis separations of the chips are investigated and the chips are fabricated by using the moulds coated with and without insulation lacquer.The performance of the chip is valuated by the determination of FITC fluorescein-labeled amino acids to a minimum detectable concentration of 0.8×10-11mol/L

when the signal-to-noise-ratio（SNR） S/N is 3.