WANG Kai1, YIN Yong-guang2. Research on application of SPR biosensor in food safety rapid assay[J]. 2007, (5): 12-14+17. DOI: 10.13873/j.1000-97872007.05.002.
SPR生物传感器在食品安全领域的应用研究
摘要
使用集成化手持式SpreetaTM SPR传感器快速检测大肠杆菌E.Coli 0157∶H7
利用大肠杆菌抗体的免疫吸附反应
采用亲和素—生物素系统放大检测的响应信号
并引入复合抗体作为第二抗体
整个检测过程在1 h内完成
实现病原微生物的快速检测。
Abstract
A rapid assay for E.Coli 0157∶H7 has been developed by using integrated portable SpreetaTM SPR biosensor.The assay is based on the immune adsorption of E.Coli antibody.Avidin-biotin system and secondary antibody have been used to achieve the rapid assay for pathogen in less than 1 h.