1. 吉林医药学院
2. 四川大学基础医学与法医学院
纸质出版:2007
移动端阅览
[1]姜艳霞,高秀峰,雷钧涛,吕士杰.毛细管DNA传感器[J].传感器与微系统,2007(02):48-49+52.
JIANG Yan-xia1, GAO Xiu-feng2, LEI Jun-tao1, et al. Capillary DNA biosensor[J]. 2007, (2): 48-49+52.
[1]姜艳霞,高秀峰,雷钧涛,吕士杰.毛细管DNA传感器[J].传感器与微系统,2007(02):48-49+52. DOI: 10.13873/j.1000-97872007.02.016.
JIANG Yan-xia1, GAO Xiu-feng2, LEI Jun-tao1, et al. Capillary DNA biosensor[J]. 2007, (2): 48-49+52. DOI: 10.13873/j.1000-97872007.02.016.
研究一种新型的利用毛细管作为载体检测靶DNA的生物传感器的可行性。首先应用0.2%多聚赖氨酸在毛细管内壁固定1×10-4mol/L ssDNA
与3.33×10-5mol/L靶DNA杂交后
应用1 g/L EB染色
在RF5000荧光仪上进行检测
t=5.5629
p<0.05
测定组与试剂空白组的荧光强度有明显差别;通过对12 h和14 h的杂交时间的比较
14 h的荧光强度明显高于12 h;对0.01
0.1
1
100
1 000 nmol/L的靶DNA进行测试
随靶DNA浓度的增高
荧光强度增强。实验结果表明:在光学DNA传感器的构建中
毛细管作为探针固定和荧光检测的载体是完全可行的。
This study investigated the feasibility of capillary as carrier for DNA detection.First
1×10-4mol/L ssDNA is fixed onto capillary inernal wall with 0.2%poly-l-lysine
then hybridizes with 3.33×10-5mol/L target DNA
and finally the ssDNA is dyed with 1g/L EB and detected by RF5000 fluorescence spectrophotometer(t=5.5629
p<0.05).The result shows that the fluorescence intensities of the samples at 14h are significantly higher than those of the blanks 12h
target DNA of 0.01
0.1
1
100
1000nmol/L is detected
and fluorescence intensity increases obviously with the increase of hybridization time.Experiments show that capillary is a practicable carrier for DNA detection.
光纤生物传感器用于核酸的特异性检测 [J]. 翟俊辉,黄惠杰,杨瑞馥,任冰强,赵永凯. 分析化学 . 2003(01)
A new method of preparing fiber-optic DNA biosensor and its array for gene detection [J] . Guangfen Jiang,Runsheng Chen,Hong Yan,Qi Ouyang. Science in China Series C: Life Sciences . 2001 (1)
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