TIAN Yan-hui, TONG Zhao-yang, LIU Bing, et al. Study on detection of Bacillus spp.target DNA sequences by piezoelectric gene sensor[J]. 2006, (5): 34-36.
TIAN Yan-hui, TONG Zhao-yang, LIU Bing, et al. Study on detection of Bacillus spp.target DNA sequences by piezoelectric gene sensor[J]. 2006, (5): 34-36. DOI: 10.13873/j.1000-97872006.05.011.
压电基因传感器检测芽孢杆菌靶序列研究
摘要
在石英晶体微天平(QCM)上用生物素亲和素法和自组装法2种不同的方法固定寡核苷酸探针
构建压电基因传感器
对芽孢杆菌靶序列进行实时检测。结果表明:生物素亲和素法固定探针效果更好
靶序列浓度为0.05
0
.5μmol/L时
有很好的线性关系
线性回归方程为Y=93.88X+10.88(R=0.989 0
N=6
P
<
0.001)
非线性误差为±4.7%。该传感器特异性较好
能够识别错配3个碱基的序列。
Abstract
Piezoelectric gene sensor which constructed with immobilizing DNA probes onto the gold electrodes of quartz-crystal microbalance(QCM) by biotin-avidin system(BAS) and molecular self-assembled monolayer(SAM)techniques is used to detect Bacillus spp.target DNA sequences in real time.The results show that BAS has better effect in probe immobilization.There is a good linear correlation between frequency decrease and target DNA concentration when it changes from 0.05
0
.5 μmol/L and the linear regression equation is Y=93.88X+10.88(R=0.989 0
N=6
P
<
0.001).The nolinear error is ±4.7 %.Specificity of the sensor is good enough to identify 3-base-mismatch DNA sequences.