A Love mode surface acoustic waves（SAW） immunosensor for staphylococcal enterotoxins B（SEB） was presented.The Love wave devices with the substrate of ST-cut quartz

interdigital transducers（IDTs） and the guiding layers of SiO

2

were fabricated successfully.The active area of sensor was precoated with γ-aminopropylethoxysilane（APTES） and glutaraldehyde

then antibody molecule probes were immobilized in a highly oriented manner by the protein A on SiO

2

surface.A system based on network analyzer was set up for conducting the immunosensing experiments.In these experiments

immobilization of antibody onto the active area of the sensor and binding with 10

-6

g/mL SEB were monitored

the phase shift of the sensor system changed obviously.The surface morphology of the active area were further characterized by using atomic force

microscopy（AFM） after protein A-based immobilization of antibodies and binding with 10

-6

g/mL SEB.