YANG Yu~1, SHI Hai-bin~1, WU Bao-yan~1, et al. Carbon nanotubes-based glucose biosensor prepared using layer-by-layer technique[J]. 2006, (1): 13-15+18.
Take multi-walled carbon nanotubes(MWCNTs) as electronic intermedia and adsorptive carrier of enzyme
a layer-by-layer self-assemble technique is used to immobilize glucose oxidase(GOx) and form(MWCNTs/GOx)
n
multilayer films to modify electrode
then a new kind of biosensor is prepared.Results show that the response current to glucose of biosensor is changed with different number of MWCNTs/GOx multilayer films
and it reaches the maximum value when the number of MWCNTs/GOx layers is 6.The response current of the(MWCNTs/GOx)
6
multilayer films-modified biosensor to 3×10
-2
mol/L glucose is 1.63 μA while the response time is only 6.7 s.The linear range of this biosensor is 5×10