WANG Shaoxi, YIN Yue, HU Chenxia. Breast cancer stem cells enrichment based on microfluidic chip[J]. 2019, (10): 73-75. DOI: 10.13873/j.1000-9787(2019)10-0073-03.
low efficiency of the traditional experimental methods
a microfluidic chip technology is proposed to study on breast cancer stem cells.A microfluidic chip for breast cancer stem cells culturing in a suspension condition with three-layer structure is designed.Cell capturing and enrichment structure is set at the middle layer.Top layer and bottom layer are used to input stem cell and culturing medium respectively and output medium individually.Experimental results demonstrate the feasibility and effectiveness of the microfluidic chip.