LI Xiufeng, FAN Beiyuan, LIU Lixing, et al. Review of quantitative detection methods of single-cell proteins[J]. 2019, (10): 1-3+7. DOI: 10.13873/j.1000-9787(2019)10-0001-03.
单细胞蛋白定量检测方法综述
摘要
单细胞水平的蛋白检测方法能够精细地反映生命事件的发生过程
同时对于少量样本的检测也至关重要。综述了不同类型的单细胞蛋白定量检测方法
包括传统技术如流式细胞术、质谱流式细胞术、酶联免疫斑点检测法和毛细管电泳法
以及基于微流控芯片的新技术。比较了这些方法的原理、主要优缺点。与传统方法相比
微流控技术能更好地操纵单细胞
检测更灵敏
在单细胞蛋白检测领域具有更大潜力。
Abstract
Single-cell protein detection methods can detailedly explain life events
at the same time
it is crucial for detection of small amounts of samples.Different types of single cell protein quantitative detection methods are reviewed
including methods based on traditional techniques such as flow cytometry
mass cytometry
enzyme-linked immunospot assay and capillary electrophoresis
and novel technology based on microfluidic chips.The principles
main advantages and disadvantages of these methods are compared.Microfluidic technology can better manipulate single cells and detect more sensitively
so it has greater potential in single cell protein detection.